[Neuroscience] Problems with transfection: eGFP - mCherry interactions?

Tom via neur-sci%40net.bio.net (by tehgabriel from web.de)
Wed Jan 14 06:04:36 EST 2009


Hi!

Did anybody ever tried co-expression of eGFP and mCherry in cultured
hippocampal neurons?

I tried this now repeatedly using CaPhos- and lipofectamine protocols
but i never found a nice red fluroescence.
No problems occured when i co-transfected eGFP and mRFP or with singel
transfection of just mCherry.

Now why won't the eGFP/mCherry co-transfection work?

Thanks for comments!

Regards,
Thomas



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