[Neuroscience] head stage

Ana Cervera Ferri via neur-sci%40net.bio.net (by Ana.Cervera-Ferri from uv.es)
Wed Jun 16 16:03:12 EST 2010


Hi all,

We have just acquired a head stage from A-M systems attached to a 1600  
Neuroprobe amplifier for extracellular single recording in  
anesthetized rats. Has any of you used it?

We still have a lot of noise in the signal, and I am not sure of how  
to use it: what should I use as a ground? should I connect a ground  
wire to the headstage or directly to the amplifier? any advice to  
obtain better recordings?

I'd qould appreciate any comment

Ana



.
El 16/06/2010, a las 19:04, neur-sci-request from oat.bio.indiana.edu  
escribió:

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>   1. Re: Different reversal potential or just poor	space-clamp?
>      (jonesmat)
>   2. Last call for applications ----- NWG-Course:	Analysis and
>      Models in Neurophysiology, Freiburg, Germany (Janina Kirsch)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 15 Jun 2010 13:56:41 -0700 (PDT)
> From: jonesmat <jonesmat from physiology.wisc.edu>
> Subject: [Neuroscience] Re: Different reversal potential or just poor
> 	space-clamp?
> To: neur-sci from net.bio.net
> Message-ID:
> 	<944dff09-c0a5-4309-b94a-f7b1edc7f6dd from c10g2000yqi.googlegroups.com>
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> On Jun 5, 12:25 am, Bill <connelly.b... from gmail.com> wrote:
>> Hi,
>> So I'm using whole-cell voltage clamp to record from a cell that has
>> two classes of inhibitory inputs. One to the soma, and one (largely)
>> to the dendrites, which I can activate independently. I/V plots show
>> me that the apparent reversal potential of the dendritic input is  
>> 10mV
>> lower than the somatic input. This could be due to the fact that a)
>> there is active Cl- homeostatis in the dendrites, which means that  
>> the
>> reversal potential truly is lower there, or b) I have poor space
>> clamp, and hence I have to drive the somatic voltage below the actual
>> reversal potential to get the potential at the dendrite to the
>> reversal potential. (or I suppose both). There is plenty of evidence
>> that I don't have proper voltage clamp of the dendritic events  
>> (slower
>> rise and decay time).
>>
>> Is there any way to figure out whether this effect is solely due to
>> cable filtering of my command voltage?
>
>
> Hi Bill,
>
> There is a way to estimate the electrotonic attenuation between your
> headstage and the synapse, though it's a little involved. It relies on
> giving voltage jumps at a series of times leading up to, and
> following, stimulation of the synapse.
>
> The method is thoroughly described here, with real data and
> simulations to test its accuracy:
>
> Estimating the time course of the excitatory synaptic conductance in
> neocortical pyramidal cells using a novel voltage jump method.
> Häusser M, Roth A.
> J Neurosci. 1997 Oct 15;17(20):7606-25.
>
> Cheers,
>
> Matt
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 16 Jun 2010 13:30:50 +0200
> From: "Janina Kirsch" <kirsch from bcf.uni-freiburg.de>
> Subject: [Neuroscience] Last call for applications ----- NWG-Course:
> 	Analysis and Models in Neurophysiology, Freiburg, Germany
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> Organisation and teaching:
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> • Dr. Ad Aertsen, Neurobiology & Biophysics, Faculty of Biology,  
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