[Neuroscience] Re: Neur-sci Digest, Vol 58, Issue 7

Hans Reiner Polder via neur-sci%40net.bio.net (by support from npielectronic.com)
Mon Mar 15 16:28:36 EST 2010


Please see
> Richter, D. W., Pierrefiche, O., Lalley, P. M. and Polder, H. R. 
> (1996). Voltage-clamp analysis of neurons
> within deep layers of the brain. J.Neurosci.Meth. 67, 121-131.
and also the attachment.

Best regards


HR Polder


neur-sci-request from oat.bio.indiana.edu schrieb:
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> Today's Topics:
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>    1. Re: Anyone do in vivo patching? (Francois Windels)
>   
>
> ------------------------------------------------------------------------
>
> Betreff:
> Re: [Neuroscience] Anyone do in vivo patching?
> Von:
> Francois Windels <f.windels from uq.edu.au>
> Datum:
> Mon, 15 Mar 2010 13:21:07 +1000
> An:
> Bill <connelly.bill from gmail.com>
>
> An:
> Bill <connelly.bill from gmail.com>
> CC:
> neur-sci from magpie.bio.indiana.edu
>
>
> Hi,
> i usually resect the meninges and then use the pressure from a 20ml 
> syringe to go down
> once on the top of my zone of interest i use only mouth pressure
> all that works well for deep brain region
>
> if you work on superficial layers of cortex you might want to use a 
> different strategy
>
> F
>
> On 12/03/2010, at 4:51 PM, Bill wrote:
>
>> Hi,
>>
>> I'm learning to do In vivo patching. However, I am getting conflicting
>> advice from my boss and from published methods/protocols papers.
>>
>> Specifically, what positive pressure do you use while penetrating the
>> meninges and unwanted structures, and what pressure do you use while
>> searching for cells in the target area?
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> ------------------------------------------------------------------------
> Francois Windels, PhD.
> Research fellow
> Synaptic plasticity laboratory
> Thinking systems project (ITEE),
> Queensland Brain Institute,
> Saint Lucia, Australia
>
>
>
>
>
>
>
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