Fw: Re: [Neuroscience] western blotting\troubleshooting

polly jo via neur-sci%40net.bio.net (by jopower from yahoo.com)
Mon Mar 29 09:55:27 EST 2010



--- On Mon, 3/29/10, polly jo <jopower from yahoo.com> wrote:

> From: polly jo <jopower from yahoo.com>
> Subject: Re: [Neuroscience] western blotting\troubleshooting
> To: Neur-sci from magpie.bio.indiana.edu, "letizia polito" <letizia.polito from gmail.com>
> Date: Monday, March 29, 2010, 8:22 PM
> 
> 
> --- On Mon, 3/29/10, letizia polito <letizia.polito from gmail.com>
> wrote:
> 
> > From: letizia polito <letizia.polito from gmail.com>
> > Subject: [Neuroscience] western
> blotting\troubleshooting
> > To: Neur-sci from magpie.bio.indiana.edu
> > Date: Monday, March 29, 2010, 2:24 PM
> >   Hi all,
> > I have some problems with WB.
> > Frequently I obtain WB with some bands missing. ((Most
> of
> > time i missed 3\4
> > bands on the right or on the left, but sometimes also
> in
> > the middle) I'm
> > pretty sure that there are not bubbles since I check
> the
> > membrane with red
> > ponceau.
> > I also thought to ECL coverage but I recently use a
> very
> > large amount of ECL
> > for 1 membrane.
> 
> We could help you better if you gave more details on the
> blotting technique you use, eg., which apparatus, wet or
> semidry; which type of paper, nitrocellulose or PVDF;
> assembly of the sandwich, buffers used, time,
> current/voltage.
> 
> Pow
> 
> > Have you got any suggestion?
> > Thanks,
> > Letizia
> > _______________________________________________
> > Neur-sci mailing list
> > Neur-sci from net.bio.net
> > http://www.bio.net/biomail/listinfo/neur-sci
> > 
> 
> 
> 
> 


      



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