[Neuroscience] Request to post
(by shabrine.daftary from tufts.edu)
Thu Nov 18 11:00:31 EST 2010
Could you please post
Can someone please tell me how they make their Cesium Gluconate internal
patch solution. I am aware that you titrate Gluconic acid with
CsOH.However, while the calculated osmolarity works out to just under
310 mOsm, the actual measured osmolarity is always much higher in the
vicinity of 355 mOsm. Objective is to record Ca currents in slices.This
should be simple enough and perhaps I will just decrease the amount of
Cs-gluconate, but any suggestions would help.
The recipe is (in mM):Cs-gluconate 120; HEPES 10; EGTA 10; CaCl2 0.5;
Mg-ATP 4; Na-GTP 0.4; TEA-Cl 20.
Thanks in advance
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