[Neuroscience] Re: Sodium imaging with SBFI

Bill via neur-sci%40net.bio.net (by connelly.bill from gmail.com)
Sun Sep 26 15:40:59 EST 2010


Hi Yishan,

This wont be much help, but I know a very experienced Ca2+ imager, who
bought some SBFI, and couldn't get it to work at all, so you're not
the only person who is having trouble with this stuff.

On Sep 25, 1:00 pm, Yishan Sun <sys... from gmail.com> wrote:
> Hi,
>
> I am trying to use the sodium indicator SBFI to measure neuronal sodium
> influx in response to depolarization. I found several references, but none
> of them precisely described a method for loading of SBFI (in the form of AM
> ester) to cultures of dissociated neurons. If you have used this technique
> in your own studies, may I learn from your experience?
>
> Have a nice weekend!
>
> Yishan



More information about the Neur-sci mailing list