[Neuroscience] P-97 Puller

Adair Oesterle via neur-sci%40net.bio.net (by adair from sutter.com)
Wed Jun 27 13:58:50 EST 2012


Concerning the P-97 Puller
This is Adair Oesterle with Sutter and it has been brought to my 
attention there is a question concerning leaving the puller on and 
concerns with "warming up" the puller.

Leaving the puller on all the time has absolutely no effect on the 
performance of the puller.
There is no needed warm up of the puller electronics or filament 
transformer and the only effect this would have on the puller
is more wear and tear on the fan and the display. So I recommend turning 
the puller off when it is not going to be used.

The only thing that really heats up on the puller are the brass jaws 
that hold onto the filament and these heat up only when you are actively 
pulling a pipette.
So, some people find that the first few pipettes are too big or not 
quite right, so they pull 2-3 pipettes, toss them and then start keeping 
the ones that were pulled after the brass jaws were warm ("warm" 
generally means between 40-60 degrees Celsius).

So if you want to warm up the jaws before pulling your pipettes, you can 
pull a few pipettes and then toss them and move onto making your 
electrodes,
or another option is to pull the bars forward, load no glass, place a 
rubber band around the puller bar knobs and press pull.
On a P-97 or a P-1000 the heat will stay on for 1.5 minutes and then the 
puller will time out and give an error message.
By doing so you can warm the jaws up before pulling the pipettes.
As long as your heat setting is at the ramp value and not far above it 
or below it, this will not damage the puller or the filament.
If you have a P-87 puller, the puller will not time out, so you should 
not use the rubber band method and instead do this by hand.

If you have a newer rev of the P-1000, the Pre-Heat mode is one of the 
NEW features in this puller. With this feature, you can go into the Menu 
of the program
and select "Pre-Heat Jaws" and once this mode has been turned on, the 
jaws will pre-heat to 70 degrees Celcius and this target temp will be 
maintained
while you are using the puller.  This will help address and remove some 
of the variability introduced from the range of temperatures of the 
brass jaws,
but if you have a stable program (by using the mid-point velocity when 
writing a patch program) you should not be experiencing variability.

Most variability has to do with:
1) The program settings are not ideal
2) NOT using the MID-POINT VELOCITY for making patch pipettes
3) The filament, air jet  and glass alignment are not correct.

Please see pages 9-16 in the Sutter Pipette Cookbook for proper 
installation of a filament.
Then follow the instructions on page 30 in the Pipette Cookbook  to 
establish the mid-point velocity and be able to write a stable program.
http://www.sutter.com/contact/faqs/pipette_cookbook.pdf

If you go through the following check list and provide the details in a 
return email to me, I can review these details and see what might be 
introducing your variability.
http://www.sutter.com/contact/faqs/variability_question_sheet.pdf

Sincerely, Adair Oesterle
415-328-6928 (cell)
415-883-0128 (office)
adair from sutter.com (email)



More information about the Neur-sci mailing list