Help with tubulin purification

Megan Oxberry oxberry at numbat.murdoch.edu.au
Wed Dec 21 22:03:36 EST 1994


I am a PhD. student in Perth, Western Australia who is trying
desperately to purify tubulin for use in an assay to measure tubulin
polymerisation.  Ultimately I would like to purify protozoal parasite
tubulin but for now I have been using lamb brain and nematode (Ascaris)
intestine as it is easier to obtain large amounts of material.  

So far I have tried using the polymerisation/depolymerisation method of
Shelanski et al. (1973) and the homogenisation, centrifugation and
dialysis method of Barrowman et al. (1984), both of which have been
unsuccessful.

Has anyone any suggestions?

Hopefully,

Megan Oxberry          oxberry at numbat.murdoch.edu.au



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