cloning of T. cruzi

Scott Davis addavis at BETA.LOYNO.EDU
Wed Oct 4 15:04:44 EST 1995


I am trying to clone various strains of T. cruzi for injection into C3H 
mice.  I keep having a problem with fungal contamination.  Recently I 
added some 5-flurocytosine to my media and have not had any success.  
Does anyone have any advice as to concentration levles of this antifungal 
or any other suggestions to prevent contamination?  ( I am using 300mg/ml).
Thanks,

Scott Davis
Loyola University
addavis at beta.loyno.edu



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