Post-doctoral Position

Jae-Seong Lee jlee at PSUHMED.RCF.HMC.PSU.EDU
Mon Oct 9 20:29:33 EST 1995


        I would be highly pleased to introduce myself to you as a post-doctoal 
fellow in Department of Pathology, College of Medicine, The Pennsylvania
State University.

        I am deeply interested in your field and so I strongly hope I could 
join in your lab as a post-doctoral fellow.

        In 1982, I entered in Department of Biology, Hanynag University, Seoul 
in Korea and learned the extensive basic biology for 4 years until 1986. 
After graduate, I enrolled in the Department of Biology (major in Cell 
Biology), the graduate school of Hayang University and I had worked in 
the field of the cellular toxicology using fish cell line, ULF-23HU 
(Umbra limi), and also had the responsibility of husbandry of 1 aquarium 
for caring about the hermaphroditic fish, Rivulus marmoratus. At that course, 
I learned the basic cellular toxicology (cytotoxicity testing, cytigenetic 
studies on sister chromatid exchanges and chromosome aberration testing), fish 
cell culture (characterization of cell line including the measurement of 
cell cycle using autoradiography and cytogentic analysis). My master works 
were published in In Vitro Cell. Dev. Biol., 25: 987-994 (1989), the abstract 
of International Icthyological Congress (The Hague, The Netherland; 1992), 
and Mutation Research, 268: 239-246 (1992).

        For 1 and half year (Sept. 1988-Feb. 1990), I served in the Army of 
Korea because this is the obligation of all men of Korea.

        In March 1990, I enrolled in the Department of Biology (major in 
Molecular Biology) of Hanyang University Graduate School. I worked on primary 
cell culture of the hermaphroditic fish, R. marmoratus for establishment of 
Rivulus cell line by August 1991. Then, I established the method of Rivulus 
cell culture, and prepared the Rivulus chromosomes for cytogenetic study.

        After September 1991, I moved to "Lab of Molecular Virology (Boss; 
Prof. Joonho Choe)", Department of Biological Science, Korea Advanced 
Institute of Science and Technology (KAIST) that is the most famous leading 
University in Korea. In there, I carried out my Ph.D. work elucidating out 
on the structure of ras genes in the hermaphroditic fish, R. marmoratus. 
At first, I made the Rivulus genomic DNA library for isolating of intact 
ras genes. I had cloned 5 kinds of different ras genes. Lately, I nominated 
them as a Rivulus Ha-, Ki-, R-ras gene, respectively. Two clones did not 
analyze yet. Rivulus Ha- and Ki-ras gene were published in two papers in 
"Biochem. Mol. Biol. Int." because they showed totally different 
characteristics in structure as compared in those of mammals. Now I am 
writing paper of Rivulus R-ras gene for submitting to "Gene". 
As you may know, R-ras gene is recently interested in the role of 
anti-oncogene and signal transduction in cell. Besides on these ras 
genes, I had cloned at least 3 different kinds of Rivulus heat shock 
protein 70 genes, and DNA repair gene (O6-alkylguanine methyltransferase 
gene). O6-MT gene is inducible gene, and involved the occurence of tumor 
by alkylating agents. Meanwhile, out team (leader; Prof. Eun-Ho Park) was 
interested in carcinogenecity of alkylating agents in R. marmoratus. So, 
we published our data on tumor incidence by alkylating agents in "Japanese 
Journal of Cancer Research". For doing more detail analysis, I analyzed 
the point mutation of Rivulus Ha- and Ki-ras gene exon 1 and 2 of alkylating
agent-induced thyroid tumor in R. marmoratus. These data were not published 
yet. In March 1994, I returned to Hanynag University for writing my thesis.

        On October 19 last year, I joined in here for extending my 
experiences in fish. Now I isolated many genes in zebrafish. My contract 
would be expired by November 15, 1995. So, I need to move another lab. 

Thanks.

        My C.V. is as follow,
---------
Personnel 
---------
Date of Birth; Sept. 28, 1963
Nationality: South Korea
Sex: Male
Marital Status: Married in 1994 (Spouse: Joo-Ok Yoon; 1  child)
Home: 15 Univeristy Manor East
      Hershey, PA 17033
      Tel & Fax: (717) 531-8447
Lab:  Department of Pathology
      College of Medicine
      The Pennsylvania State University
      Hershey, PA 17033
      Tel. (717) 531-4704
      e-mail: jlee at cor-mail.biochem.hmc.psu.edu

-----------
Education
-----------
1982-1986       Department of Biology, Hanyang Unviersity, Seoul, Korea. B.S.
1986-1988       Department of Biology, Hanyang Unviersity Graduate School, Seoul
                Korea. M.S. Supervisor: Prof. Eun-Ho Park
1990-1994       Department of Biology, Hanyang Unviersity Graduate School, Seoul
                Korea. Ph.D. Supervisor: Prof. Eun-Ho Park

------------
Experiences
------------
1988-1990       Military Service, The Army of Korea
1990-1991       Research Assistant, Research Institute of Environmental
Sciences,
                Hayang University, Seoul, Korea
1991-1992       Research and Teaching Assistant, Dept. of Biology, Hanyang
                University, Seoul, Korea
1994-Present    Post-doctoral Fellow, Dept. of Pathology, College of Medicine
                The Pennsylvania State University, Hershey, Pennsylvania
                Supervisor: Asst. Prof. Keith C. Cheng

--------------------
List of publications
-------------------- 

1. Lee, J.-S., 1988. Growth kinetics and cytogenetic characteristics of the
fish cell line, ULF (Umbra limi fin)-23HU. M.S. dissertation, Hanyang 
Univeristy, Seoul, Korea. pp. 1-34.

2. Park, E.-H., J.-S. Lee, A.-K. Yi and H. Etoh, 1989. Fish cell line 
(ULF-23HU) derived from the fin of the central mudminow (Umbra limi):
suitable characterstics for clastogenecity assay. In Vitro Cell. Dev. Biol.,
25: 987-994. (USA)

3. Park, E.-H., Y. J. Kim, D. H. Byun, J.-Y. Lee and J.-S. Lee, 1992. baseline 
frequency of sister chromatid exchanges in 142 persons of the general Korean 
population. Mutat. Res., 268: 239-246. (The Netherlands)

4. Lee, J.-S., 1994. Structure of the ras oncogenes in the hermaphrodic fish,
Rivulus marmoratus. Ph.D. dissertation, Hanyang Unviversity, Seoul, Korea.
pp. 1-104.

5. Lee, J.-S., J. Choe and E.-H. Park, 1994. Absence of the intron-D-exon of 
c-Ha-ras oncogene in the hermaphrodic fish Rivulus marmoratus (Teleostei: 
Rivulidae). Biochem. Mol. Biol. Int., 34: 921-926. (Australia)

6. Lee, J.-S., J. Choe and E.-H. Park, 1995. Genomic structure of the
c-Ki-ras proto-oncogene of the hermaphroditic fish Rivulus marmoratus
(Teleostei: 
Rivulidae). Biochem. Mol. Biol. Int., 35: 57-63. (Australia)

7. Lee, J.-S., J. Choe and E.-H. Park, 1995. Isolation and characterization of 
R-ras gene in the hermaphroditic fish Rivulus marmoratus. In Preparation.

-----------
References
-----------

1. Prof. Eun-Ho Park
Department of Biology
College of Natural Sciences
Hanyang University
Seoul 133-791, South Korea
Tel. +82-2-290-0953
Fax  +82-2-299-3495

2. Asst. Prof. Keith C. Cheng
Department of Pathology
College of Medicine
The Pennsylvania State University
Hershey, PA 17033
Tel. (717) 531-5635
Fax  (717) 531-5021

3. Asso. Prof. Joonho Choe
Department of Biological Science
Korea Advanced Institute of Science and Technology
Taejon 305-701, South Korea
Tel. +82-42-869-4020
Fax  +82-42-869-4010

Thank you for your consideration.


Best wishes,

Jae-Seong Lee, Ph.D.
Department of Pathology
College of Medicine
The Pennsylvania State University
Hershey, PA 17033





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