fixing crypto to slides

wick at cris.com wick at cris.com
Thu Dec 26 15:14:44 EST 1996


I'm having a difficult time fixing crypto oocysts to microscope slides. I have used a standard procedure of air drying 
purified oocysts, fixing with 95% ethanol, washing with PBS, followed by application of the antibody per IFA. 
Apparently all the oocysts are washed away.

Question: what procedures do others use to directly fix crypto to microscope slides?

Jim Wick

E-mail:		wick at cris.com		
Phone:		414-871-7199
FAX:		414-871-1273
Snail Mail:	5520 W. Burleigh St.
		Milwaukee, WI 53210

"Don't precipitate more than you can resuspend"






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