How to do bacteria cultures

m.reinen n2aih at planet.net
Sun Jan 14 19:05:19 EST 1996


CINDY wrote:
> 
>      I wish to conduct a project by culturing bacteria taken
> from people with sore throats. However, I do not know the best
> method of extracting the bacteria. Do I use a cotton swab to
> get the bacteria from the throats or do I ask the volunteer to
> gargle his/her mouth with water and use the water to do
> the culture. Also I wish to know any traditional medicines one
> will reccommend for sore throats. Any suggestions??
> Thanks. Any help will be much appreciated.
> Cindy.Using  a sterile swab swipe the swab over the back of the throat and the 
side (near the tonsils) usually I use a circular motion. the culture 
media should be able to isolate gram positive organisms and gram negative 
organisms ie t soy blood agar and  emb or mackonkeys agar  

good luck Mark F Reinen MT



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