Trichomonas vaginalis

[Lance C. Russell]

AntiCancer wrote:
> 
> To whom it may concern,
> 
>  Can anybody help me out with a protocol on how to prepare a culture of
> Trichomanas vaginalis for storage in liquid N2? I assume, the methods
> are different from those used in bacteria and mammalian cells. Any
> suggestions are greatly appreciated!
> 
>  thanks in advance, sincerely,
> 
>  M.Lenz

Check this reference:

Diamond LS, Bartgis IL, and Reardon LV.  1965.  Virulence of Trichomonas
vaginalis after freeze-preservation for two years in liquid nitrogen
vapor.  Cryobiology 1(4):295-297.


Lance