Long PCr primers

Wellcome Trust, Nairobi. wellcome at USERS.AFRICAONLINE.CO.KE
Sat Nov 8 08:40:24 EST 1997


> 
> Dear Colleagues,
> 
> If there any risk of amplifying a fragment DNA with primer which are 50 to 60 pb. Actually, I want to introduce a enzyme restriction site in these primers so that I can easily  detected them in agore or Acrylamide gel. Therefore I do need longer primers. But I wandering about doing a PCR such long oligonucleotides.
> My regards,

-- 
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