Conformational epitopes in Toxoplasma gondii membrane proteins p30 and p22.

Recobinant Antigens agrec at cigb.edu.cu
Wed Oct 7 11:09:57 EST 1998


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We had cloned and expressed p30 and p22 T. gondii membrane proteins in =
E. coli. Both are expressed in high levels and recognized in Western =
blot by some positive IgG human sera.=20
We want to know if the main epitopes in those proteins are =
conformational?=20
If it is so, if there are a solution to this potential problem?
We are trying to obtain proteins that are recognized by all the sera =
positive to T. gondii in humans.
=20
Thanks in advance,
Marcelo Nazabal Galvez
Molecular Biology Lab.
e-mail: agrec at cigb.edu.cu

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<DIV><FONT color=3D#000000 size=3D2>We had cloned and expressed p30 and =
p22 T.=20
gondii membrane proteins in E. coli. Both are expressed in high levels =
and=20
recognized in Western blot by some positive IgG human sera. =
</FONT></DIV>
<DIV><FONT color=3D#000000 size=3D2>We want to know if the main epitopes =
in those=20
proteins are conformational? </FONT></DIV>
<DIV><FONT color=3D#000000 size=3D2>If it is so, if there are a solution =
to this=20
potential problem?</FONT></DIV>
<DIV><FONT color=3D#000000 size=3D2>We are trying to obtain proteins =
that are=20
recognized by all the sera positive to T. gondii in humans.</FONT></DIV>
<DIV><FONT color=3D#000000 size=3D2></FONT>&nbsp;</DIV>
<DIV><FONT color=3D#000000 size=3D2>Thanks in advance,</FONT></DIV>
<DIV><FONT color=3D#000000 size=3D2>Marcelo Nazabal Galvez</FONT></DIV>
<DIV><FONT color=3D#000000 size=3D2></FONT><FONT size=3D2>Molecular =
Biology=20
Lab.</FONT></DIV>
<DIV><FONT color=3D#000000 size=3D2>e-mail: <A=20
href=3D"mailto:agrec at cigb.edu.cu">agrec at cigb.edu.cu</A></FONT></DIV></DIV=
></BODY></HTML>

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