PCR Resource Site
Steven B. Harris
sbharris at ix.netcom.com
Wed Sep 30 02:46:41 EST 1998
In <6ur26a$7ga at sjx-ixn2.ix.netcom.com> gmc0 at ix.netcom.com (George M.
>Gregory LaMont Casey <gcasey at postoffice.pacbell.net> wrote:
>>As far as the PCR is concerned, I would also suggest that you go to
>>site of the man that won the Nobel Prize for it, Kary Mullis, Ph.D.
>>yahoo or any other web server and look him up. I was told that he
>By all means.
>>Another benefit would be to read about his views regarding HIV and
>>of the PCR. He stated in an interview that I watched, that the PCR
>>be used for determining viral loads. You will never get accurate
>>According to Dr. Mullis, the numbers you will get will always be
>Well, this is true--it's an amplification process! This seems to be
>backpedaling on Mullis's part. Indeed, PCR is routinely used to
>measure viral load in blood. It has limitations, to be sure, but the
>correlation between higher viral load and advancement of disease
>(whether HIV or HCV) is very strong.
> George M. Carter
PCR does indeed give true values. Tests are run against a standard
curve of known dilutions of the sequence in question. If the test
gives correct answers for knowns, what justification has Mullis for
thinking it doesn't for unknowns? He must be off his rocker.
Steve Harris, M.D.
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