Fractionating Leishmania

Aravind Kumar aravind at
Mon Jan 31 02:28:28 EST 2000

Hi, I am working on leishmania cells. 
I am trying to fractionate the cells, and i am specially interested in the
mitochondrial and the nuclear fraction. I am having problems in purifying
them, especially nuclei.

Does any one has any experiance.

Thanks very much for any reply. 

Sperm biotechnology, 
National Institute of Immunology, 
New Delhi.


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