[Parasitology] Urgently looking for specimens of human whipworm (Trichuris trichiura)

Damiana Ravasi via parasite%40net.bio.net (by damianaravasi from gmail.com)
Mon Dec 15 04:23:14 EST 2008


Dear all,

My name is Damiana Ravasi, I am a PhD candidate collaborating with Dr
Nichola Illing (Dept of Molecular Biology at UCT) on a project to
investigate whether baboons on the Cape Peninsula share the same parasites
as humans.
We have successfully amplified the ITS1 and ITS2 regions from genomic DNA
from one Trichuris worm isolated from a baboon gut, using the NC2 and NC5
primers (Cutillas et al 2002 Parasitol Res 88: 574-582)...but have not been
able to identify the corresponding sequence for Trichuris trichiura from
Genbank. We have tried to obtain a human Trichuris trichiura locally, but
without success. Next best would be to try and get the sequence of Trichuris
trichuira from humans from another source.

*Has anyone ever managed to sequence the human Trichuris trichiura ITS1 and
ITS2?
Or would anyone have an aliquot of Trichuris trichiura genomic DNA or the
nematode specimens which you would willing to send to us?*

Thank you very much
Damiana Ravasi

-- 
Damiana Ravasi

UCT Baboon Research Unit (BRU)
Zoology Department
University of Cape Town
Rondebosch 7701
South Africa

Tel: +27 (0)21 6503645
Fax: +27 (0)21 6503295
Cell: +27 (0)72 1908305
E-mail: damianaravasi from gmail.com
http://www.baboonsonline.com/bru/project/parasites
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