<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><!--StartFragment--><p class="MsoNormal" style="margin-bottom:12.0pt;text-align:justify">Dear All,</p><p class="MsoNormal" style="margin-bottom:12.0pt;text-align:justify"><span lang="EN-GB">Cryptonet (<a href="http://www.cryptonet.ie/">www.cryptonet.ie</a>)
is pleased announce a three day practical workshop on molecular techniques and
risk assessment focused on<span style="mso-bidi-font-weight:bold"> surface
water contamination by <i style="mso-bidi-font-style:normal">Cryptosporidium spp.
</i>from agricultural sources. </span><span style="mso-spacerun:
yes"> </span>All are welcome to join at University College Dublin,
Belfield, Dublin, Ireland from <strong>Wednesday 3<sup>rd</sup> to Friday 5<sup>th</sup>
June 2009.<span style="mso-spacerun: yes"> </span></strong><strong><span style="font-weight:normal;mso-bidi-font-weight:bold">The workshop will be </span></strong>hosted
by the UCD School of Agriculture, Food Science and Veterinary Medicine, and The<b style="mso-bidi-font-weight:normal"> </b><strong><span style="font-weight:normal;
mso-bidi-font-weight:bold">Central Veterinary Research Laboratory (DAFF)</span></strong><b style="mso-bidi-font-weight:normal">. </b>Participants can choose to join just
the one day symposium on molecular methods and risk assessment and, if they
wish one of two follow on workshops detailed below.</span></p><p class="MsoNormal" style="margin-bottom:12.0pt;text-align:justify"><!--StartFragment--><p class="MsoNormal" align="center" style="text-align:center"><!--[if gte vml 1]><v:group
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<strong><u><span lang="EN-GB" style="font-size:14.0pt;font-family:"Times New Roman";
mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman";
color:blue;mso-ansi-language:EN-GB;mso-fareast-language:EN-GB"><p class="MsoNormal" style="margin-bottom:12.0pt;text-align:justify"><br></p></span></u></strong></p>
<pre style="text-align:justify"><font class="Apple-style-span" color="#0000FF" face="'Times New Roman'" size="4"><span class="Apple-style-span" style="font-size: 16px;"><br></span></font></pre><pre style="text-align:justify"><font class="Apple-style-span" color="#0000FF" face="'Times New Roman'" size="4"><span class="Apple-style-span" style="font-size: 16px;"><br></span></font></pre><pre style="text-align:justify"><font class="Apple-style-span" color="#0000FF" face="'Times New Roman'" size="4"><span class="Apple-style-span" style="font-size: 16px;"><span class="Apple-style-span" style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; white-space: normal; "><p class="MsoNormal" align="center" style="text-align: center; font-size: 17px; "><b><span lang="EN-GB" style="font-variant: small-caps; color: blue; ">Contamination of surface water by <i>Cryptosporidium </i>spp.</span></b></p><p class="MsoNormal" align="center" style="text-align: center; font-size: 17px; "><b><span lang="EN-GB" style="font-variant: small-caps; color: blue; ">A workshop on molecular biological techniques and risk assessment</span></b></p></span></span></font></pre><pre style="text-align:justify"><span lang="EN-GB" style="font-size:12.0pt;
font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman";
color:blue">A one day </span><span style="font-size:12.0pt;font-family:"Times New Roman";
mso-bidi-font-family:"Times New Roman";color:blue;mso-ansi-language:EN-US;
mso-fareast-language:EN-US">symposium</span><span style="font-size:12.0pt;
font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman";
mso-ansi-language:EN-US;mso-fareast-language:EN-US"> bringing together methodologies in </span><span lang="EN-GB" style="font-size:12.0pt;font-family:"Times New Roman";mso-bidi-font-family:
"Times New Roman"">molecular identification of <i style="mso-bidi-font-style:
normal">Cryptosporidium</i> spp. and microbial risk assessment will be held on <strong>Wednesday June 3<sup>rd</sup> 2009</strong>.<span style="mso-spacerun: yes"> </span>A combination of international and local speakers will present on key research issues relating to using molecular tools and risk assessment for <i style="mso-bidi-font-style:normal">Cryptosporidium</i> spp. in agricultural catchments.<span style="mso-spacerun: yes"> </span>Attendance at the symposium is welcome without participation in the concurrent practical workshops to be held on June 4<sup>th</sup>-5<sup>th</sup> 2009.<span style="mso-spacerun: yes"> </span><o:p></o:p></span></pre><pre style="text-align:justify"><span lang="EN-GB" style="font-size:12.0pt;font-family:
"Times New Roman";mso-bidi-font-family:"Times New Roman""><span style="mso-spacerun: yes"> </span><o:p></o:p></span></pre><p class="MsoNormal" style="margin-bottom:6.0pt;text-align:justify"><b style="mso-bidi-font-weight:normal"><span lang="EN-GB" style="color:blue">Workshop
1:</span></b><span lang="EN-GB"> Molecular Techniques</span></p><p class="MsoNormal" style="margin-bottom:12.0pt;text-align:justify"><span lang="EN-GB">A two-day practical workshop on molecular biological techniques:
towards the development of standard protocols for the detection and
quantification of <i>Cryptosporidium</i><span style="mso-bidi-font-style:italic">
</span>oocysts in various environmental matrices. This session will cover the
DNA extraction from complex environmental matrices (soil, slurry and
contaminated water) and the detection of <i style="mso-bidi-font-style:normal">Cryptosporidium
spp.</i> using real time qPCR techniques. Special attention will be dedicated
to statistical analysis of real time PCR data.</span></p><p class="MsoNormal" style="margin-bottom:6.0pt;text-align:justify"><b style="mso-bidi-font-weight:normal"><span lang="EN-GB" style="color:blue">Workshop
2:</span></b><span lang="EN-GB"> Microbial Risk Assessment</span></p><p class="MsoNormal" style="text-align:justify"><span lang="EN-GB">A one-day
workshop on risk assessment techniques applied to the study of non point source
agricultural pollution. Using a participatory-based approach to teaching and
learning, attendees will have the opportunity to use software tools and contribute
to in-depth discussions, share experiences, and exchange resources in a variety
of thematic topics such as Microbial and Ecological Risk Assessment, Risk Based
Site Evaluation, <i style="mso-bidi-font-style:normal">Cryptosporidium</i>
Epidemiology.<span style="mso-spacerun: yes"> </span>Participants will
develop a sound understanding in developing conceptual risk assessment models
and semi-quantitative methods in describing microbiological risk.</span></p><p class="MsoNormal" style="margin-bottom:12.0pt;text-align:justify"><span lang="EN-GB"><o:p> </o:p></span></p><p class="MsoNormal" style="margin-bottom:12.0pt;text-align:justify"><span lang="EN-GB">Registration form could be found at <a href="http://www.cryptonet.ie/workshop09/">www.cryptonet.ie/workshop09/</a> <span style="mso-spacerun: yes"> </span></span></p><p class="MsoNormal" style="text-align:justify"><span lang="EN-GB"><o:p> </o:p></span></p><p class="MsoNormal" style="text-align:justify"><span lang="EN-GB">To request more
information contact: <a href="mailto:marco.berzano@ucd.ie">marco.berzano@ucd.ie</a>
or <a href="mailto:peter.ziegler@ucd.ie">peter.ziegler@ucd.ie</a> </span></p><p class="MsoNormal" style="text-align:justify"><br></p><p class="MsoNormal" style="text-align:justify">Apologies for cross-posting.</p>