NIH Membrane Protein Crystallization Workshop

Fri Jul 2 09:35:53 EST 1993

                             WORKSHOP ANNOUNCEMENT


                              August 16-17, 1993

                         National Institutes of Health
                        Building 31C, Conference Room 6
                              Bethesda, Maryland

The objectives of the meeting will be to determine:

1) the state of the art in the field of membrane protein crystallography,
2) the state of the art in NMR spectroscopy of membrane proteins,
3) the feasibility of achieving atomic resolution structures for the
   respiratory enzymes within the next five to ten years,
4) obstacles and opportunities toward achieving that goal, and
5) models for productive research collaboration on these problems.

The scope of the meeting will include an overview of work on all integral
membrane proteins and the problems common to determining their structures.
The meeting will then focus on the following systems:

1) mitochondrial electron transport proteins and their microbial counterparts,
2) plant and bacterial photoreaction centers and electron transport chains,
3) ATP synthases and other ion-motive ATPases, and
4) membrane transport proteins required for oxidative and photosynthetic

The meeting will feature a series of overview lectures during the morning of
the first day to present the state of the art in five key scientific areas.
The same topics will be revisited in the afternoon by panel discussions
devoted to updates and a critical review of these areas.  The second day will
be devoted to developing specific recommendations to stimulate work in this
area.  A report of the workshop and its recommendations will be presented to
the NIGMS Advisory Council.  Refer to the following Workshop Agenda for

You are invited to attend this workshop and to contribute to the panel
discussions and recommendations development session.  Registration is required
since the number of attendees that can be accommodated is limited.  No
registration fee is involved.  Please return the enclosed Meeting Reservation
Form if you wish to attend.  We have reserved a block of rooms at the Bethesda
Hyatt Regency Hotel near the NIH Campus.  Let us know if you plan to stay at
this hotel and we will be pleased to arrange your reservations.
Unfortunately, we will not be able to provide financial support for your
attendance at this meeting.

If you will not able to attend, but would like to comment on the potential for
stimulating research in this area, please write to me directly.  I will make
your letter available to the workshop participants and include a consensus of
these letters in the report to the NIGMS Advisory Council.Sincerely yours,

Peter C. Preusch, Ph.D.
Program Administrator
Cellular and Molecular Basis
 of Disease Program
National Institute of General Medical Sciences
National Institutes of Health
Westwood Building, Room 906
5333 Westbard Avenue
Bethesda, Maryland, U.S.A.  20982

Phone:  301-594-7806
FAX:    301-594-7728
E-Mail: peter_preusch at nihgmsww.bitnet                           MEETING RESERVATION FORM

                   Structural Biology of Respiratory Enzymes
                 Crystallography and NMR of Membrane Proteins
                              August 16-17, 1993
                         National Institutes of Health
                              Bethesda, Maryland

Please print or type clearly and complete all sections of the form.
We will be pleased to make reservations for you at the Bethesda Hyatt Regency
Hotel.  Note that without a credit card guarantee, reservations at the Hyatt
will only be held until 4:00 p.m.  You are of course free to use other
accommodations.  However, please return this form even if you will not need
room reservations.

Please call Ms. Hope Kott if special accommodation will be needed either at
the meeting or at the hotel.  Phone:  (301)-594-7837.

Name ________________________________________________________

Address ______________________________________________________



Phone # ______________________   Fax # ______________________

I will arrive on August ________  at ________ a.m. or p.m.

I will depart on August  ________ at ________ a.m. or p.m.

I would like a _______ single ______ double _______ no smoking

_______  handicapped equiped room.

Special meeting rates are $110 single, $138 double.  All room rates are
inclusive of 12% occupancy tax.

I will use my _________________ credit card to guarantee my reservation.

The number is __________________________________ expiration date _________.

Name as it appears on the card ___________________________________________.

Fax or mail to:   Ms. Hope Levy Kott
                  National Institute of General Medical Sciences
                  Westwood Building Room 9A09
                  Bethesda, Maryland  20892

FAX: (301) 594-7701
                                WORKSHOP AGENDA

MONDAY, August 16, 1993

Morning Session:  8:30 a.m. - 12:30 p.m.

Introductory Comments and Welcome
Ruth Kirschstein, Director, National Institute of General Medical Sciences
Peter Preusch, Program Administrator, Bioenergetics Portfolio, Cellular and
   Molecular Basis of Disease Program, NIGMS

Keynote Speech:  Approximately 1/2 hour.
"On the value of structure determination in elucidating function.  A case
study: the bacterial photoreaction center".
   George Feher (University of California-San Diego)

Topic Overviews:  Approximately 30 min each, including discussion.
Session Co-Chairpersons: Ronald Kaback (University of California-Los Angeles)
                         Norma Allewell (University of Minnesota)

1.  Membrane Protein Crystallography - Work in Progress
      Johann Deisenhofer (University of Texas Southwestern Medical Center)

2.  Protein Production Issues - Cloning/Expression/Purification
      Shelagh Ferguson-Miller (Michigan State University)

3.  Crystallization Methodology
      Michael Garavito (University of Chicago)

4.  Methods of Structure Solution for 2D and 3D Crystals
      Robert Glaeser (University of California-Berkeley) - 2D crystals
      Elinor Adman (University of Washington) - 3D crystals

5.  Applicability of Nuclear Magnetic Resonance Methods
      Robert Griffin (Massachussets Institute of Technology)

Lunch:  NIH Cafeteria

Afternoon Session:  1:30 p.m. - 5:30 p.m.

Panel Discussions and Critical Reviews:  Approximately 45 minutes each.

1.  Membrane Protein Crystallography - Work in Progress: Updates + Critique
      Chairperson:  William Cramer (Purdue University)
      Panelists:    Mario Amzel (Johns Hopkins), Janet Smith (Purdue), Johann
      Deisenhofer (UTSWMC).

      Suggested questions to seed the discussion:
      a.  How many structures have been solved?
      b.  How many efforts are on-going?
      c.  How close are they to achieving atomic resolution?
      d.  What are the present obstacles and opportunities?
      e.  What is the present level of financial support for this work?

2.  Protein Production Issues - Cloning/Expression/Purification
      Chairperson:  Robert Gennis (University of Illinois)
      Panelists:    Bernard Trumpower (Dartmouth), Shelagh Ferguson-Miller
      (Michigan State U.)., Peter Pedersen (Johns Hopkins), Tomoko Ohnishi (U.
      Pennsylvania), James Fee (Los Alamos Natl. Lab), Ronald Kaback (UCLA).

      Suggested questions to seed the discussion:
      a.  Which enzymes from which sources show the most promise?
      b.  What further developments in molecular genetics are needed?
      c.  What further developments in detergent technology are needed?
      d.  What opportunities exist for producing soluble protein fragments?

3.  Crystallization of Membrane Proteins
      Chairperson:  Enrico Stura (Scripps Research Institute)
      Panelists:    Michael Garavito (U. Chicago), Mary Lyon (U. Colorado),
      George Feher (UC-San Diego).

      Suggested questions to seed the discussion:
      a.  What methods are available for crystallization?
      b.  What methods are available for heavy atom derivatization?
      c.  What types of interactions stabilize membrane protein crystals?
      d.  What novel methods can we imagine that are not currently in use?

4.  Solution of Protein Structures
      Chairperson:  Norma Allewell (University of Minnesota)
      Panelists:    Johann Deisenhofer (UTSWMC-Dallas), Robert Glaeser
      (Lawrence Berkeley Lab), Mario Amzel (Johns Hopkins), Elinor Adman (U.
      Washington), Janet Smith (Purdue).

      Suggested questions to seed the discussion:
      a.  Are the present methods adequate for proteins of this complexity?
      b.  What methods are available to handle specific types of disorder?
      c.  What are the resolution limits of structural work on 2D crystals?

5.  Potential for NMR methods
      Chairperson:  Robert Fillingame (U. Wisconsin)
      Panelists:    Robert Griffin (MIT), Timothy Cross (Florida State U.),
      Adriaan Bax or Stephan Grzesiek, Marius Clore, Angela Gronneborn (NIH)

      Suggested questions to seed the discussion:
      a.  What is the status of the field for membrane proteins?
      b.  Is there any specific obstacle to solving membrane structures?

Dinner:  Informal group dinner at a local restaurant.

Evening Writing Session: Individual and group efforts at the hotel.TUESDAY, AUGUST 17, 1993

Morning:  8:30 a.m. - 12:00 p.m.

Session Co-Chairpersons:  Frank Prendergast (Mayo Clinic)
                          Peter Pedersen (Johns Hopkins University)

Reports of Monday Session Chairs
  Further discussion and amendment by panels.

Discussion Leading to Workshop Recommendations
Panelists:  All workshop participants.

Suggested questions for discussion.

1.  Is there a need to stimulate more work in this area?
2.  What are the highest priority areas for support?
3.  What level of additional support would be beneficial?
4.  What forms of stimulation are likely to be productive?
5.  Workable models for collaborative research?

Break for Lunch:  12:00

Discussion to continue in the afternoon.

Meeting to adjourn by 3:00 p.m.

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