Chloroplast Suspension Protocol?

[singsaas at students.wisc.edu]

Ben Levin <benjinsl at astro.ocis.temple.edu> wrote:
>

> 
>  I'm looking for a protocol for separating chloroplasts (centrifugation,
> yes?) 
> 

I've had good luck with the method of Keegstra et al. (sorry, I don't have
the reference handy).  It uses a Percoll gradient and is fairly quick.
-------------------------
Eric L. Singsaas
UW-Madison Dept. of Botany