Need protocol on Caged cAMP

Dr. L. Darjania darjania at criba.edu.ar
Tue Jul 2 13:11:52 EST 1996


Dear Group Members

Currently I am working on cAMP mediated signaling in guard cell
protoplasts of Vicia faba. Particularly I am interesting in
cAMP regulated protein kinases and would like to test a possible
alteration in protein phosphorylation after [cAMP] increases in cytosol
using caged cAMP.

Regarding to this, could anybody send me a protocol dealing to loading of
plant protoplasts with caged cAMP, and relevant callibration.

Thanks

Dr. L. Darjania
Dept. de Agronomia
Universidad Nacionasl Del Sur
Bahia Blanca 8000, Argentina

e-mail: darjania at criba.edu.ar





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