Couple seeks two postoc positions in plant science/molecular biolog=

daemon at net.bio.net daemon at net.bio.net
Mon Sep 30 14:42:12 EST 1996


To whom it may concern:

In the moment, I and my girlfriend are looking for postdoctoral positions
in plant science/molecular biology, starting by December 96 - February
97. Now we are both working in the Institute of Plant Genetics and Crop
Plant Research, Gatersleben, Germany and we are prepared to relocate, but
only together.
We both have experience in molecular biology, PCR methods, gene cloning
and expression analysis, expression of proteins in E.coli, transformation
of plants etc.. Furthemore we can guarantee an excellent cooperation
between us and also with other scientists.

Our resumes follow.

Thank you in advance.

RNDr Stanislav Kopriva
Dept. of Molecular Cell Biology
Institute of Plant Genetics and Crop Plant Research
06466 Gatersleben
Germany



Curriculum Vitae 1:
RNDr Stanislav Kopriva, born on March 13, 1967 in Ceske Budejovice, Czech
Republic
present adress: Department of Molecular Cell Biology
                Institute of Plant Genetics and Crop Plant Research
                06466 Gatersleben
                Germany
Phone:   +49-39482-5363
Telefax:  +49-39482-5366
E-mail:   kopriva at ipk-gatersleben.de

Education:
Charles University, Department of Biochemistry, Prague 1985-1990
- Graduation with honors, M.Sc., 1990
- Dean=B4s Award for Outstanding Results, 1990
- Diplom Thesis: Interaction of cytochrom P-450IIB1 with
fluoresceinisothiocyanate
- Research Assistentship, Dept. of Biochemistry, 1987-1989
Grammar School Prague, 1981-1985
- Student research work: Influence of forest age on the amount of
nitrogen in water, 1985

Employment:

Research fellow, Department of Molecular Cell Biology, Protein
Biochemistry group,  Institute of Plant Genetics and Crop Plant Research,
Gatersleben, Germany, 1996-now
- research on Calvin cycle enzymes pentose phosphate-3-epimerase and
transketolase, sequence analysis,     expression analysis, expression in
E. coli, in vitro mutagenesis, study of the catalytic mechanism, analysis
  of anti-epimerase transgenic plants.

Postgraduate Student, Department of Molecular Cell Biology,
Photosynthesis group, Institute of Plant Genetics and Crop Plant
Research, Gatersleben, Germany, 1992-1995
- Ph.D. Thesis: The glycine decarboxylase multienzyme complex of
=46laveria: Expression study and phylogenetic   analysis, submitted to
Martin Luther University Halle, May 1996 expected time of defense -
November 96
- research on expression of GDC in plants with different types of
photosynthesis (C3, C3-C4, and C4), sequence   analysis of cDNAs encoding
GDC, and phylogenetic analysis of the Flaveria genus based on cDNA
sequences of   H-subunit of GDC.
- published 12 articles and 8 poster presentations (list of publication
included)

Research Fellow, Department of Biochemistry, Charles University, Prague
1990-1991
- research on cytochrom P-450: isolation from rats, rabbits, and carps,
selective FITC labelling, peptid   mapping, enzymatic analysis
- supervising undergraduate practical training


Main research achievements:
- Isolation and sequence analysis of cDNA clones for three subunits of
glycine decarboxylase and serine   hydroxymethyltransferase and
comparison of their expression in Flaveria species with different types
of   photosynthesis
- Identification and characterization of alternative splicing of
H-protein pre-mRNA in C4  species of Flaveria
- Characterization of tissue-specific expression of multiple genes coding
for P-protein and H-protein of GDC in   Flaveria pringlei.
- The first evidence of phylogenetically intermediate position of C3-C4
intermediate photosynthesis
- Isolation and characterization of cDNA clones coding for
pentosephosphate-3-epimerase and transketolase   multigene family. This
is the first eukaryotic epimerase and the first transketolase from higher
plants
- expression of active PPE in E. coli, in vitro mutagenesis, isolation of
mutated proteins, analysis of the   enzyme catalytic activity, proposal
of catalytic mechanism.

Research skills:
cDNA library construction and screening, manual sequencing, sequence
analysis, DNA, RNA, and protein isolation, Southern, Northern, and
Western blotting, PCR, RT-PCR, competitive PCR, molecular cloning,
preparation of constructs for protein expression in E. coli and for plant
transformation, analysis of transgenic plants, in vitro mutagenesis,
phylogenetic analysis, HPLC, et cetera.

Research interests:
Photorespiration, evolution of C4 photosynthesis, phylogenetic analysis,
control and mechanism of alternative splicing, regulation of cell-type
specific gene expression, RNA editing, cytochrome P-450, cancerogenesis,
cell development

Other skills:
good active knowledge of four languages: Czech, English, Russian, and
German


List of publications

=B7 Kopriva S. and Bauwe H.: T-protein of Glycine Decarboxylase from
Solanum tuberosum. Plant Physiol. 104:   1079-1080 (1994)
=B7 Kopriva S. and Bauwe H.: P-protein of Glycine Decarboxylase from
=46laveria pringlei. Plant Physiol. 104:   1077-1078 (1994)
=B7 Kopriva S. and Bauwe H.: Serine hydroxymethyltransferase from Solanum
tuberosum. Plant Physiol. 107: 271-272   (1995)
=B7 Bauwe H. and Kopriva S.: The gdcsPA Gene from Flaveria pringlei
(Asteraceae). Plant Physiol. 107: 655 (1995)
=B7 Kopriva S., Turner S.R., Rawsthorne S. and Bauwe H.: T-protein of the
Glycine Decarboxylase Multienzyme   Complex: Evidence for partial
similarity to formyltetrahydrofolate synthetase. Plant Mol. Biol. 27:
1215-1220   (1995)
=B7 Kopriva S., Cossu R., Bauwe H.: Alternative splicing results in two
different transcripts for H-protein of   the glycine cleavage system in
the C4 species Flaveria trinervia. Plant J. 8(3): 435-441 (1995)
=B7 Bauwe H., Chu C.-C., Kopriva S., Nan Q.: Complete structure and
expression analysis of the gdcsPA and gdcsPB   genes encoding two
P-isoproteins of the glycine cleavage system from Flaveria pringlei. Eur.
J. Biochem. 234:   116-124 (1995)
=B7 Kopriva S. and Bauwe H.: H-protein of glycine decarboxylase is encoded
by multigene families in Flaveria   pringlei and F. cronquistii
(Asteraceae). Mol. Gen. Genet. 249: 111-116 (1995)
=B7 Teige M., Kopriva S., Bauwe H., S=FCss K.-H.: Chloroplast
pentose-5-phosphate 3-epimerase from potato: Cloning,   cDNA sequence,
and tissue-specific enzyme accumulation. FEBS Lett. 377: 349-352 (1995)
=B7 Kopriva S., Cossu R., Chu C.-C., Bauwe H.: Tissue specific alternative
splicing of H-protein of glycine   decarboxylase in C4 Flaveria species.
In: Mathis P. (ed) Photosynthesis: from Light to Biosphere, Vol III,
655-658 (1995)
=B7 Kopriva S., Chu C.-C., Bauwe H.: H-protein of the glycine cleavage
system in Flaveria: Alternative splicing   of the pre-mRNA occurs
exclusively in advanced C4 species of the genus. Plant J. 10: 369-373
(1996)
=B7 Kopriva S., Chu C.-C., Bauwe H.: Molecular phylogeny of Flaveria as
deduced from the analysis of H-protein   nucleotide sequences. Plant Cell
Environ. in press (1996)
=B7 Teige M., Kopriva S., Bauwe H., S=FCss K.-H.: Molecular cloning,
sequencing, and expression analysis of   chloroplast transketolase from
potato. Evidence that oxidative pentose phosphate cycle does not exist in
  non-photosynthesising plant organs. J. Biol. Chem. submitted

Lectures

=B7 Kopriva S.: =84Phylogenetic analysis of Flaveria based on H-protein cDNA
sequences=93, IPK Gatersleben, 10.4.1995
=B7 Kopriva S.: =84The glycine decarboxylase multienzyme complex from
=46laveria: Expression study and phylogenetic   analysis=93, Institute of
Plant Science, ETH Z=FCrich, 12.8.1996


Posters

=B7 Kopriva S., Chu C., Deng Z., Cossu R., Bauwe H.: Glycine Decarboxylase
complex in Flaveria: A comparison of   gdcH genes in species with
different degrees of C4   photosynthesis.  Gordon Research Conference -
CO2
  fixation, October 1993, Irsee
=B7 Kopriva S., Bauwe H.: Glycine decarboxylase complex in plants:
Molecular cloning and primary structure of the   T-protein from P.
sativum, S. tuberosum, and F. pringlei.  Gordon Research Conference - CO2
fixation, October   1993, Irsee
=B7 Kopriva S., Cossu R., Bauwe H.: cDNA cloning and analysis of gcsH,
gcsP, gcsT, and shmt genes from Flaveria   species with different types
of photosynthesis. 7.Tagung =84Molekularbiologie des Pflanzen=93, March 1994=
,
  Wernigerode
=B7 Kopriva S., Cossu R., Bauwe H.: Glycine decarboxylase in Flaveria.
Arbeitstagung des SFB, May 1994, Halle
=B7 Bauwe H., Kopriva S.: Glycine decarboxylase from plants: Cloning of the
gdcsPA gene from Flaveria pringlei, a   C3 species and some properties of
the corresponding protein. 4th International Congress Plant  Molecular
Biology, June 1994, Amsterdam
=B7 Chu C., Cossu R., Kopriva S., Deng Z., Bauwe H.: A comparison of GDCSH
genes from Flaveria. 4th International   Congress Plant Molecular
Biology, June 1994, Amsterdam
=B7 Kopriva S., Cossu R., Bauwe H.: Glycine decarboxylase multienzyme
complex from Flaveria, Arbeitstagung des   SFB, May 1995, Halle
=B7 Kopriva S., Cossu R., Chu C.-C., Bauwe H.: Tissue specific alternative
splicing of H-protein of glycine   decarboxylase in C4 Flaveria species.
Xth International Photosynthesis Congress, August 1995, Montpellier


List of referees:

Dr. Hermann Bauwe
Biochemistry and Physiology Department
IACR-Rothamsted
Harpenden, Herts. AL5 2JQ, UK
Phone:     01582-763133
Telefax:   01582-760981
E-mail:     hermann.bauwe at bbsrc.ac.uk

Dr. Karl-Heinz S=FCss
Dept. of Molecular Cell Biology
Inst. of Plant Genetics and Crop Plant Research
06466 Gatersleben
Germany
Phone:  039482-5363
Telefax: 039482-5366

Dr. Steve Rawsthorne
Dept. of Brassica and Oilseed Research
Cambridge Laboratory
John Innes Centre
Norwich NR4 7UH, UK
Phone:   01603-452571
Telefax:  01603-259882
E-mail:   steve.rawsthorne at bbsrc.ac.uk




CURRICULUM VITAE 2

Name: Anna V. Zavgorodnyaya, Ph.D.
Place of Birth: Lutsk, Volyn Province, UKRAINE.
Date of Birth: October 28, 1967.
Nationality: Russian.
Marital status: divorced.

Address for correspondence:
Dr. Anna V. Zavgorodnyaya
Department of Molecular Cell Biology
Institute of Plant Genetics and Crop Plant Research
06466 Gatersleben
Germany
Phone:   +49-39482-5318
Telefax:  +49-39482-5366
E-mail:     zavgorod at ipk-gatersleben.de



Employment:

1995- present:      Institute of Plant Genetics and Crop Plant Research,
Gatersleben, Germany
                             research fellowship.
                             - regulation of chlorophyll biosynthesis
                             - effect of herbicide treatment
                             - expression in E. coli and in vitro
mutagenesis of ALA synthase

1991 - 1995:         Institute of Cell Biology and Genetic Engineering,
                              National Academy of Science of Ukraine,
Kiev, UKRAINE.
                               Scientific Researcher.
                             - Ph. D. Thesis: =84Nucleo-cytoplasmic
inheritance in asymmetric somatic
                               hybrids in Lycopersicon genus".


Education:

1985-1990           Department of Genetics, Kiev State University, Kiev,
UKRAINE.
                            - 1990 - M.Sc. Genetics.
                            -  Diplom Thesis: =84Effect of DNAseI treatment
on different evolutionary stadia
                               of Drosophilla=93.
                            -  Scientific Researcher Assistant, Institute
of Cell Biology and Genetic
                               Engineering, National Academy of Science
of Ukraine, Kiev, 1988-1990





Main research achievements:

- Complementation of GSA-AT antisense mutations by transformation with
yeast ALA synthase
- expression of active ALA-S in tobacco plants
- production of gabaculline tolerant tobacco plants
- investigation of influence of gabaculine and ALA on photosynthesis and
expression of related proteins
- first complex analysis of inheritance of organell and nuclear encode
genes in fertile asymmetric somatic   hybrids between Lycopersicon
esculentum and L. peruvianum.
- evidence for rearrangements in chloroplast genome of this hybrids

Research skills:

Southern, Northern, Western analysis; DNA, RNA, protein isolation;
molecular cloning, restriction analysis; preparation of constructs for
protein expression and for antisense and sense plant transformation;
analysis of transgenic plants; PCR; in vitro mutagenesis; enzyme assays;
isolation of organells from plants; work with radioisotops and in vitro
cultures; somatic hybridization; isolation of chloroplast and
mitochondrial DNA

Scientific interests:

Chlorophyll biosynthesis and its regulation; relation of protein
structure and function; effect of herbicides on plants; genetic
ingeneering; genetic transformation of plants; recombination of
cytoplasmic genomes; nucleo-cytoplasmic interractions; RNA editing in
plant organells; cancerogenesis.

Languages:

English, Russian, Ukrainian - read / speak.

 List of publications

Ratushnyak YI, Piven NM, Latypov SA, Samoylov AM, Zavgorodnyaya AV, Gleba
YY (1991) Changes of the genetic constitution of the tomato plants by
gamma-fusion of Lycopersicon esculentum Mill. and Lycopersicon peruvianum
var. dentatum Dun. protoplasts. Biopolymers and Cell  7:82-91.

Ratushnyak YI,  Cherep NN,  Zavgorodnyaya AV,  Latypov SA, Borozenko  IV,
 Rachkovskaya  RI,  Gleba  YY  (1993)  Cell engineering synthesis of
tomato fertile asymmetric somatic hybrids by gamma-fusion of protoplasts.
 Genetika,  29:164-176.

Ratushnyak YI,  Cherep NN,  Zavgorodnyaya AV,  Latypov SA, Borozenko IV,
 Rachkovskaya RI,  Gleba YY  (1993)  Fertile asymmetric somatic hybrids
between Lycopersicon esculentum  Mill. and Lycopersicon peruvianum var.
dentatum Dun. Mol. Gen. Genet, 236: 427-432.

Rudas  VA,  Zavgorodnyaya AV,  Cherep NN,  Gleba YY (1993) Genetic
transformation by leaf-disks method of wild species Solanum and
Lycopersicon. Biopolymers and  Cell  9:73-77.

Ratushnyak YI, Kochevenko SA, Cherep NN, Zavgorodnyaya AV, Latypov SA,
Gleba YY (1995) Alloplasmic incompatibility of cybrid plants possest
nuclear Lycopersicon esculentum and plasmagenes of Lycopersicon
peruvianum var. dentatum Dun. Genetica 31(5): 660-667.

Zavgorodnyaya AV  (1994) Nucleo-cytoplasmic inheritance in asymmetric
somatic hybrids in Lycopersicon genus. Ph.D. thesis, Kiev, UKRAINE.

Cherep NN, Zavgorodnyaya AV, Ratushnyak YI, Gleba YY (1995)
Reconstruction of chloroplast DNA in asymmetric somatic hybrids
Lycopersicon esculentum Mill. + Lycopersicon peruvianum var. dentatum
Dun.  Genetica  (in press).

Zavgorodnyaya AV, Grimm B (1996) Yeast 5-aminolevulinate synthase
provides additional chlorophyll precursor in transgenic tobacco
(submitted EMBO J.).

Zavgorodnyaya AV, N.N., Grimm B (1996) Influence of gabaculline on
expression of proteins involved in chlorophyll biosynthesis and
photosynthesis (manuscript in preparation).

ABSTRACTS:

Piven NM, Ratushnyak YI, Rudas VA, Latypov SA, Zavgorodnyaya AV,  Gleba
YY  (1991)  Selection  of  somatic  hybrids in  Lycopersicon and Solanum
using chlorophyll-deficient mutants and gamma-irradiation.  Abstr  VIII
Internl  Protoplasts Symposium, Uppsala, p 28.

Cherep  NN,  Zavgorodnyaya  AV,  Ratushnyak  YI,  Gleba YY    (1993)
Chloroplast  DNA  rearrangement in Lycopersicon asymetric somatic
hybrids.  Abstract  XVIIth  Intern.  Congress of Genetics, Birmingham, p
191.

Ratushnyak YI,  Cherep NN,  Zavgorodnyaya AV,  Latypov SA,  Kochevenco
AS, Gleba YY (1994) Alloplazmic incompatibility in cybrid plant of tomato
obtained by cell engineerihg methods.  Abstract VIIIth Intern. Congress
of Plant Tissue and Cell Culture, Firenze, p 101.

Cherep NN,  Zavgorodnyaya  AV,  Ratushnyak  YI,  Gleba  YY  (1994)
Organell and nuclear genomes analysis of tomato cybrid plants.  Abstracts
4th Intern.  Congress  of  Plant  Molecular Biology, Amsterdam, p 153.


LECTURES:

ETH Z=FCrich, 12.8.1996: =84Yeast 5-aminolevulinate synthase provides
additional chlorophyll precursor in transgenic tobacco=93



References:

Grimm Bernhard, Dr. hab.
Department of Molecular Cell Biology
Institute of Plant Genetics and Crop Plant Research
06466 Gatersleben
Germany
Tel.:  49-39482-5364
=46ax:  49-39482-5139
E-mail: grimm at ipk-gatersleben.de

Gleba, Y.Y., Professor, Ph.D.
Director of Institute of Cell Biology and Genetic Engineering,
American Cyanamid Company,
Princeton, NJ 08540,
U.S.A.
Tel: 1-609-799-0400.
=46ax: 1-609-799-1842.
E-mail: glebay at pt.cyanamid.com

Alisher Touraev, Ph.D.
Institute of Mikrobiology and Genetics,
Biocentre of Bohrgasse, Vienna University
Dr.Bohrgasse 9
1030 Vienna
Austria.
Tel: 1-79515 7820.
=46ax: 1-798 62 24.





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