Hello and DNA isolation

Dr. David Starrett c309scb at SEMOVM.SEMO.EDU
Fri Oct 27 14:31:21 EST 1995


Plant-ed compatriots,

  I am a new subscriber to the group.  Apologies if the following has
recently been asked, discussed, etc.  Does anyone have a good protocol for
extracting DNA from any plant material which doesn't require centrifugation
OR phenol, chloroform or other nsty chemicals (EtBr O.K.).  Situation:
University doesn't have a low speed centrifuge now.  High speed not
available for teaching.  Teachin "lab" has no fume hoods, etc.  I am
teaching Plant Physiology to upper division students.  They would like to
extract plant DNA.  I am trying to figure out a way to let them achieve this
within the constraints of our equipment, etc.  Any suggestions much appreciated.

Thank You,

David Starrett
Biology Department, MS 6200
Southeast Missouri State Univeristy
Cape Girardeau, MO 63701
Ph: (314) 651-2382
Fax: (314) 651-2223
email: c309scb at semovm.semo.edu




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