sib at brown.edu
Mon Oct 30 09:52:15 EST 1995
<Pine.SUN.3.91.951030083545.514B-100000 at london.biology.lsa.umich.edu>,
ldnum at BIOLOGY.LSA.UMICH.EDU (Larry D Nooden) wrote:
> My vote is for phaeophytin. Basically what happens is that acids
> released from the vacuoles during homogenization of the cells cause the
> Mg+2 in chlorophyll to be replaced with H+, so you will need to use a
> strong buffer ca. pH 7. See Canfield et al. in Annals of Botany 75: 143-
> (1995) for discussion and references.
Although pheophytin is likely to be formed during extraction of acidic
plant cells, I doubt that it is the pigment under consideration, because
pheophytin is more hydrophobic than chlorophyll and would therefore run
ahead of chlorophyll rather than stay behind.
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