mysterious pigment

Sam Beale sib at
Mon Oct 30 09:52:15 EST 1995

In article
<Pine.SUN.3.91.951030083545.514B-100000 at>,
ldnum at BIOLOGY.LSA.UMICH.EDU (Larry D Nooden) wrote:

> My vote is for phaeophytin.  Basically what happens is that acids 
> released from the vacuoles during homogenization of the cells cause the 
> Mg+2 in chlorophyll to be replaced with H+, so you will need to use a 
> strong buffer ca. pH 7.  See Canfield et al. in Annals of Botany 75: 143- 
> (1995) for discussion and references.

Although pheophytin is likely to be formed during extraction of acidic
plant cells, I doubt that it is the pigment under consideration, because
pheophytin is more hydrophobic than chlorophyll and would therefore run
ahead of chlorophyll rather than stay behind.

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