plant for tissue culture

Ross Koning Koning at ECSUC.CTSTATEU.EDU
Fri Sep 22 08:03:47 EST 1995


Almost any solanaceae you have in the greenhouse would
be a great prospect.  If your garden isn't through, that
would include some tomato plant stems and petioles, green
pepper, maybe even petunia.  If your gardens are done
for the year, then here is an idea I have used many
times.  Try sunflower seeds.  Of course you need to use
the fresh, unprocessed (unfried!) seeds.  I generally get
a fresh bag of sunflower oil seeds (sold as outdoor bird
feed) and get great germination in the agar.  As the
seedling grows, you get wonderful callus development
in the hypocotyl.  Soybeans, and a range of grain seeds
work too.  The trouble with grains is lots of nooks and
crannies to surface sterilize.  Use 10% Clorox for at
least 10 minutes, and a spritz of 95% alcohol as a surface
wetting agent beforehand does not hurt.

Good luck!

ross

At  8:05 PM 9/21/95 -0700, bio_jxn at shsu.edu wrote:
>Can someone tell me a good/easy plant for an intro plant physiology
>lab concerning tissue culture, other than tobacco?
>Since the tobacco seeds I recently planted
>have not germinated and the lab is in three weeks, I am looking for
>an alternative.  Any suggestions of plants or plant parts which can
>be purchased from a grocery store or something I can grow from seeds
>in three weeks, would be greatly appreciated. Thank you.
>Joan Hudson
>Dept. of Biological Sciences
>Sam Houston State University
>Huntsville, TX
>bio_jxn at shsu.edu

Ross Koning
Biology Department                     **n**
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