plant media

Ross Koning Koning at ECSUC.CTSTATEU.EDU
Mon Oct 7 08:10:34 EST 1996


At  5:51 PM 10/6/96 -0700, Michael Joseph Kerkman wrote:
>I made some medium with people in my college class, we added agar and
>autoclaved. But after it cools its supposed to gell. It didn't. Anybody
>know why? Thanks

We might need some more information.  What amount of
agar was dissolved in what volume of medium?  How
long did you wait before deciding it would not gel?
What were the components of the medium?  Did you
adjust the pH correctly?  Did you adequately mix
the medium and agar before and after autoclaving?
These are all critical elements to deciding on how
to proceed.

ross

______________________________________________________________
                            |
Ross Koning                 | Koning at ecsu.ctstateu.edu
Biology Department          | http://koning.ecsu.ctstateu.edu/
Eastern CT State University | Phone: 860-465-5327
Willimantic, CT  06226  USA | Fax: 860-465-5213
____________________________|_________________________________

                Plant Physiology is Phun!

 /\|___/\     //\______COOH   NH-CH2-CH=C-CH2OH  \/OH
|  |  |  |    |  |  ||       //\___     \CH3     /\|/\\/\\COOH
 \/ \/|\/|    \\/ \ /       N  ||  N            |  |
 /\ | |__|=        NH       |  || ||           //\//\
  | COOH                    \\ /\ /            O
  COOH        H2C=CH2         N  NH
______________________________________________________________





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