Root protoplasts--Help!!!

DAVID SERRANO - EL MARIACHI. dserrano at csupomona.edu
Mon Jun 30 16:31:05 EST 1997


We are currently trying to derive protoplasts from Avocado roots.  We have
tried high doses of of Cellulase and Macerase (~0.1%) to no avail.  We have
also tried searching the literature for protocols, but have found none.  If
anyone is aware of a protocol for deriving protoplasts from roots it would
greatly be appreciated.  Any and all responses can be posted here or e-mailed
to DSerrano at CSUPomona.edu.

			Thanks in Advance,

				David.



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