Toluidine Blue

Nancy Harrison vulpia at
Tue Oct 7 19:12:01 EST 1997

I need a recipe for this stain, to be used to stain
fresh plant tissue. I know several people have mentioned
it here - we don't have any at our lab, although we could
order it from Sigma. We're not sure whether to use the
alcohol solution or the aqueous solution. We'd rather
just get the raw materials and mix it ourselves.
  We've had fun this past week with students being able
to "slice and dice" supermarket vegetables to look at
primary stem structure, but our stains leave a lot
to be desired! -NH

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