cDNA library needed for a course

Jon Monroe monroejd at jmu.edu
Wed Jul 22 10:08:21 EST 1998


Plant-eders,

I'm teaching Molecular Biology in the fall and we've been using an
Arabidopsis cDNA library for a semester-long project in which students
isolate plasmid DNA from random colonies, construct maps, sequence the
ends, then attempt to identify them using BLAST.
[http://ip25-204.pc.jmu.edu/DOCS/mbio/mblab/mbpage1.html]  The project
works well but, because the Arabidopsis genome is being sequenced, the odds
of finding something novel are getting worse and worse.  What we would like
is a new cDNA library preferable from an odd organism (not well studied at
the molecular level and not necessarily a plant).  We would also prefer
that it be in ZipLox vector because plasmids are very easy to rescue.  If
you or a colleague have such a library and would be willing to share,
please contact me (monroejd at jmu.edu).

Thanks!

Jon

-------------------------------------------------
  Jonathan Monroe
  Associate Professor
  Department of Biology
  James Madison University
  Harrisonburg, VA 22807
  voice:  540-568-6649 (office)
          540-568-6045 (lab)
  fax:    540-568-3333
  e-mail: monroejd at jmu.edu
  www.jmu.edu/biology/biofac/jmonroe/jmonroe.html
-------------------------------------------------





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