Fixing Sunflower Stems

Monique Reed monique at bio.tamu.edu
Mon Oct 26 13:38:52 EST 1998


You can try fixing in formalin and then moving to alcohol or an 
alcohol-ethylene-glycol mix.  That might work.

M. Reed

>Can anyone suggest an alternative to FAA (formalin-acetic
>acid-alcohol) as a fixative for plant stem sections.   

>I have some two-foot-high sunflower plants growing under growth
>chamber conditions and intended for free-hand stem sectioning and
>staining in a botany lab on stem anatomy, scheduled in about three
>weeks.   By then, the plants will have passed the point of being best
>for sectioning, so I would like to cut the stems into 1-1.5-inch
>sections and submerge them in fixative for the 3 week period.   From
>what I read, FAA would serve the purpose, but I would like to know if
>there is a non-formalin based fixative that would work as well or
>better so we can avoid the formalin.   Suggestions on any part of this
>plan, particularly the fixative, will be appreciated.

>John E. Silvius
>Professor of Biology
>Box 601
>Cedarville College
>Cedarville, Ohio   45314
>E-mail:   silviusj at cedarville.edu 
>http://www.cedarville.edu/dept/sm/jes_www.htm




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