Pollen tubes

Janice M. Glime jmglime at MTU.EDU
Mon Apr 5 22:36:18 EST 1999

I have had pretty good luck with pollen from Easter lilies on plain agar
with the pollen spread about 1 mm from stigma tissue on the agar.  sugar
water works with some (10-30%) - I usually try whatever is blooming in the
greenhouse and give it 2-3 hours or more to germinate. > 
> How easy is it to demonstrate pollen tube formation in a lab?  What kinds
> of pollen work best?  What problems and difficulties are encountered? 
> Could you point me toward a procedure for doing this?  
> Thanks.
> John
> jbohmfal at hastings.edu

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