Growing Arabidopsis?

Grant R. Cramer cramer at UNR.EDU
Mon Aug 2 09:41:37 EST 1999


Dave,
I don't think it is wise to grow Arabidopsis without aeration. Many plants
will grow "OK" without aeration in hydroponics. I have been using
hydroponics for my salinity research since 1980. Oxygen will diffuse
adequately in the upper couple of inches, but once they grow deeper and
depending on the volume of roots to the volume of solution, the oxygen will
become depeleted rapidly. For optimal growth you need to aerate the
solution. If you are conducting experiments, there is no point in risking
interactions with anaerobiosis. I am sorry I missed the poster at the ASPP
meetings!
--
Grant R. Cramer
Associate Professor
Mail Stop 200
Department of Biochemistry
University of Nevada
Reno, NV 89557
phone: (775) 784-4204
fax: (775) 784-1650
email: cramer at med.unr.edu
web page: http://BIOCHEM.MED.UNR.EDU/faculty/grant_c/

----------
>From: drobinson at bellarmine.edu ("Robinson, Dr. David")
>To: plant-ed at net.bio.net
>Subject: RE: Growing Arabidopsis?
>Date: Sun, Aug 1, 1999, 12:08 PM
>

> Bob:  At last week's ASPP meeting there was an interesting poster on growing
> Arabidopsis hydroponically. It was poster # 47 by R.N. Arteca (abstracts are
> online).
>
> I am going to try it: float foam-rubber (they looked like the kind used in
> Drosophila vials) stuck into the holes of test-tube racks floating in a 1/8
> nutrient solution. Place the seeds on top of the foam, and they grow down
> into it. Aeration is not required if you change the solution once a
> week....the roots grow right down into it.
>
>              Dave Robinson, Biology, Bellarmine College
>
>> -----Original Message-----
>> From: cramer at unr.edu [SMTP:cramer at unr.edu]
>> Sent: Thursday, July 29, 1999 8:24 PM
>> To: plant-ed at net.bio.net
>> Subject: Re: Growing Arabidopsis?
>>
>> I think the best way to grow Arabidopsis is to grow it hydroponically. We
>> have found this plant to be very sensitive to the slightest overwatering
>> or
>> underwatering in the earling seedling stage. We grow them on rock wool
>> system that we have developed. It is idiot proof. Rockwool is the key! See
>> my paper on the internet
>> at:http://BIOCHEM.MED.UNR.EDU/faculty/grant_c/hydroponic.html
>> --
>> Grant R. Cramer
>> Associate Professor
>> Mail Stop 200
>> Department of Biochemistry
>> University of Nevada
>> Reno, NV 89557
>> phone: (775) 784-4204
>> fax: (775) 784-1650
>> email: cramer at med.unr.edu
>> web page: http://BIOCHEM.MED.UNR.EDU/faculty/grant_c/
>>
>> ----------
>> >From: monroejd at jmu.edu (Jon Monroe)
>> >To: plant-ed at net.bio.net
>> >Subject: Re: Growing Arabidopsis?
>> >Date: Thu, Jul 29, 1999, 9:58 AM
>> >
>>
>> > Bob,
>> >
>> > We grow Arabidopsis plants in 5" plastic pots.  Eight pots are held
>> > in 22" x 11" trays and 12 trays fit on a growth cart on wheels.  I've
>> > purchased all of these items from a place called Grower's Supply in
>> > Ann Arbor MI (313-426-5852).  Another source of Arabidopsis-specific
>> > growing supplies is Lehle Seeds (http://www.arabidopsis.com/).  I'm
>> > sure there are other good suppliers out there.  I fill the trays
>> > 1/3-1/2 full with nutrient solution at planting and then add tap
>> > water when they run out - about every third day initially and almost
>> > every day when they get larger.
>> >
>> > I had the same problem with seedling death-for-no-apparent-reason
>> > earlier this year so I checked the archives of the Arabidopsis
>> > newsgroup (http://www.bio.net/hypermail/ARABIDOPSIS/) and learned
>> > that this is a somewhat common problem!  No one appears to have has
>> > spent the time to figure out the cause, but the most frequent advice
>> > I saw was to switch soil brands.  I was using Pro-Mix BX when the
>> > problem started so I tried Sunshine Mix and MetroMix and plants grew
>> > normally on both of them.  Interestingly, people had the same problem
>> > with a variety of soil mixes so when it happens again I'll switch
>> > back!
>> >
>> > Hope this helps.
>> >
>> > Jon
>> >
>> >
>> >>Does anyone have a good system for growing Arabidopsis?  Mine get to the
>> >>seedling stage but then die because I can't seem to keep them from
>> getting
>> >>either too dry or too wet.
>> >>
>> >>TIA
>> >>
>> >>Bob
>> >>Dr. Robert R. Wise
>> >>Department of Biology
>> >>University of Wisconsin Oshkosh
>> >>Oshkosh, WI 54901
>> >>(920) 42403404
>> >>wise at uwosh.edu
>> >>http://www.uwosh.edu/departments/biology/wise/wise.html
>> >
>> >
>> > ---------------------------------------------------
>> >   Jonathan D. Monroe           Associate Professor
>> >   Department of Biology       office: 540-568-6649
>> >   MSC 7801                       lab: 540-568-6045
>> >   James Madison University       fax: 540-568-3333
>> >   Harrisonburg, VA 22807   email: monroejd at jmu.edu
>> >   http://csm.jmu.edu/biology/monroejd/jmonroe.html
>> > ---------------------------------------------------



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