Nancy S. Kirkpatrick nkirkpatrick at LAKERS.LSSU.EDU
Thu Feb 4 11:36:55 EST 1999

I found this recipe for Toluidine blue O.  Add 0.05 g toluidine blue O
to 100 ml of 0.1M phosphate buffer, pH 6.8.  Dilute this mixture 1:1
with distilled water before use.

Nancy S. Kirkpatrick, PhD.
Assistant Professor &
Chief Health Professions Advisor
Biology Department
Lake Superior State University
Sault Ste. Marie, Mi  49783
(906) 635-2894 FAX: (906) 635-2266
nkirkpatrick at

Jerry Pickering wrote:
> Does anyone know the correct means of preparing Toluidine Blue stain?
> If so, please post it.  I thank you for your assistance regarding this
> request.


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