Autoclaving Hormones vs Filter Sterilizing
Carol M. Stiff
kck at TURBONET.COM
Sat Feb 27 11:39:43 EST 1999
An alternative to filter sterilization of the GA is to dissolve it in DMSO, and add it to the
medium after autoclaving. DMSO sterilizes the chemical thus eliminating the need to filter
sterilize. Make the GA up at 10 mg/ml and this will allow you to use a minimum of DMSO in the
medium. Your typical explants tolerate this well (buds, nodes, leaf tissue, etc.) but the DMSO
could be problematic to individual cell or protoplasts so be cautious with its use.
Carol M. Stiff, Ph.D, President and CEO
Kitchen Culture Kits, Inc.
818 Ford Street, Moscow, Idaho 83843
President, Stiff Applied Technology, Inc.
Forest Inventory Consulting/Webpage Development
Commissioned Sales Associate/Technical Advisor for PPM
Plant Cell Technology, Inc.
| From: Ross Koning <koning at ecsuc.ctstateu.edu>
| To: plant-ed at net.bio.net
| Subject: Autoclaving Hormones vs Filter Sterilizing
| Date: Friday, February 26, 1999 2:08 PM
| At 4:46 PM -0500 2/26/99, Bob Wise wrote:
| >To all,
| >We have some tissue culture set-ups to which we wish to add GA and CK. Can
| >these hormones be autoclaved or do they have to be filter sterilized?
| Benzyl Adenine (BAP) can be autoclaved with little
| loss of activity. I am under the impression that
| GA is not particularly labile either...but I haven't
| done that personally. Frankly, though, with the
| sterile disposable filters, adding these to cooling
| media after autoclaving and prior to dispensing is
| not too difficult or horrendously expensive. You
| can run them through the filter at the same time
| to save on cost.
| Ross Koning | koning at ecsu.ctstateu.edu
| Biology Department | http://koning.ecsu.ctstateu.edu/
| Eastern CT State University | phone: 860-465-5327
| Willimantic, CT 06226 USA | fax: 860-465-4479
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