Nicotiana growth conditions

Grant R. Cramer cramer at MED.UNR.EDU
Wed Oct 13 12:21:19 EST 1999

We grow our plants in growth chambers at 16 h days, 28 C day temp, 23 C 
night. Many of the tobacco varieties used today are day neutral, meaning
daylength won't affect flowering. We grow them hydroponically with our
Gibeaut's solution (see
Grant R. Cramer
Associate Professor
Mail Stop 200
Department of Biochemistry
University of Nevada
Reno, NV 89557
phone: (775) 784-4204
fax: (775) 784-1650
email: cramer at
web page:

>From: koning at (Ross Koning)
>To: plant-ed at
>Subject: Re: Nicotiana growth conditions
>Date: Wed, Oct 13, 1999, 8:30 AM

> At 11:45 AM -0500 10/12/99, Kelly McConnaughay wrote:
>>Dear Plant-edders
>>I am a student at Bradley University beginning an undergrad research
>>project.  I will be comparing the induced responses of Nicotiana sylvestris
>>to simulated herbivory after grown in low and high nitrogen availability
>>and ambient and elevated CO2 atmospheres.  But I need to know what are
>>normal greenhouse growing conditions for Nicotiana sylvestris (soil
>>composition, temp, everything) and the range of nitrogen availability for
>>these plants in the field?
>>Any info is appreciated,
>>Julie Lasanen
>>please send replies to kdm at
> Hi Julie!
> I'm growing a lot of N. sylvestris in my
> greenhouses here at ECSU. The plants are
> thriving for me.  I am growing them in 4.5 inch
> Kord geranium pots (sorry, English standards for
> pots). I am using Fafard #2 potting soil (not
> too different from ProMix BX). The plants are
> watered twice daily with a drip irrigation system
> with what I hope is 100 ppm N from a 20-20-20
> Peters soluble fertilizer mix. At present I am
> leaving the walkway fluorescent lights on 24hours
> a day so that the plants are under long-day
> conditions to flower.  I am trying to get seeds
> from crosses of these transgenic plants. My
> temperature settings are 75 day/65 night. I am
> not supplementing with CO2 at all other than
> what visiting humans give off.  The greenhouse is
> a two-layer polyethylene hoop house, and I have
> 55% shade cloth over it at present but it is
> coming off soon.  The growth is lush in the
> partial shade.
> Best wishes!
> ross
> ________________________________________________________________
> Ross Koning                 | koning at
> Biology Department          |
> Eastern CT State University | phone: 860-465-5327
> Willimantic, CT 06226 USA   | fax: 860-465-4479
> ____________________________|___________________________________
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