Staining phloem

Frederique Guinel fguinel at wlu.ca
Fri Sep 24 07:55:19 EST 1999


	The recipe I used for aniline blue is the following: 0.05% w/v
water-soluble aniline blue in 0.067M phosphate buffer, pH=8.5.
	It is a fluorescent dye, and its fluorescence emission peak is 509
nm.  The stained sections should be looked at under UV light.  
	It is rather specific for beta(1,3)glucans, such as callose.    
	This stain can be added to a section previously stained with PAS. 
This allows a student to recognize the tissue under bright-field.  The
stain does not need to be rinsed.  One adds a drop on top of the section
and put directly a coverslip on top.

	Good luck Magaly!
	Frederique.
	
Frederique C. Guinel
Assistant Professor
Department of Biology
Wilfrid Laurier University
Waterloo, ON, N2L 3C5, Canada
(519) 884-0710  Ext 2230
FAX (519) 746-0677

On 23 Sep 1999, Bob Vickery wrote:

> Magaly Rincon-Zachary wrote:
> 
> >Does anybody know of an easy staining procedure to visualize sieve tube
> >members in fresh tissue?
> >
> >Thank you very much.
> >
> >Magaly
> 
> 
> 
> There is a stain that stains callose a bright blue.  From memory, which may be wrong, the recipe is 0.1% (or was it 0.01% ?) aniline blue in a bicarbonate buffer at pH 8.  The aniline blue turns to brown over a couple of days, so the solution should be made up shortly before use from a concentrated solution of aniline blue in ethanol.
> 
> Cheers
> 
> Bob Vickery
> vickery at mpx.com.au
> 
> 




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