starch assay

Mark D. Spiro spiro at
Fri Jan 28 09:46:19 EST 2000

Grant Cramer wrote:
>Can anyone give the details on the use of KI for a starch assay?

The basics of the KI starch assay involve the following solution according
to "Experiments in Plant Physiology" by Carol Reiss (what a great book!).

5g KCl
0.36g KIO3
in 1L 2mN NaOH

This solution mixed with soluble starch will yield a blue color.   A at 580nm
is proportional to [starch].

Mark D. Spiro
Department of Biology
Bucknell University
Lewisburg, PA 17837
spiro at
phone:  (570) 577-3486
fax: (570) 577-3537


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