DNA transfer techniques

[sjohnson at mtsu.edu]

agrobacterium system was developed and initially worked with 
dicots only. so it's probably not the mechanism for bt corn, unless 
new inovations have made agbact/monocot interactions possible.

sandra

> Dear Plant Edders,
> 
> Does anyone know the method of DNA transfer of the gene of interest into Bt
> Maize, Roundup-Ready Oil Seed Rape (Canola) and Flavr-Savr tomatoes? Any
> other examples would also be of interest.
> 
> Agrobacterium? Ballistics? Electroporation? ....?
> 
> The question came up during discussion with some of my students and I had
> to confess I didn't know the answer but was confident that I could find out
> via Plant-Ed!
> 
> Thanks in advance.
> 
> Barry
> 
> Dr.Barry Meatyard
> Science and Plants for Schools
> Environmental Sciences Research and Education Unit
> Warwick Institute of Education
> University of Warwick
> Coventry
> CV4 7AL
> UK
> 
> Email: barry.meatyard at warwick.ac.uk
> Tel: 44 (0) 2476 524228
> Fax: 44 (0) 2476 523237
> 
> SAPS is at http://www-saps.plantsci.cam.ac.uk
> 
> 
> ---


*************
Sandra L. Johnson, Ph.D.
Plant Physiological Ecologist
Middle Tennessee State University
Biology Department     PO Box 60
Murfreesboro, TN  31732

Phone: (615) 898-2021
FAX: (615) 898-5093
---