andrew.bettany at bbsrc.ac.uk
Fri Jan 26 03:39:57 EST 2001
I can't answer your question directly, but I can help you work it out!
You can calculate centrifugal force using the following formula:
g = 1.12 x r (max radius of rotor in mm) x [(rpm/1000) squared]
rpm = 1000 x square root (g / 1.12 r)
Hope this helps,
Dr. Andy Bettany
Institute of Grassland and Environmental Research
"Fisher, Roxanne" wrote in message
<239A087D853BD31198BC00062938073B02813F77 at exchange.chatham.edu>...
>Dear Plant-ed folks:
>I want to do a photosynthesis lab with isolated thylakoid membranes for my
>Plant Physiology class. I'm thinking of something like the lab in
>"Experiments in Plant Physiology" by Carol Reiss. Her protocol calls for
>spinning the crushed and processed spinach at 1900 g to pellet the
>thylakoids. At Chatham we only have a small centrifuge that goes to
>2500rpm. Will spinning in this centrifuge be strong enough to pellet the
>thylakoids? We do have microfuges that are fast enough but I'd like to
>avoid dividing the sample up into tiny aliquots if possible.
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