Leaf clearing for microscopy
gcote at radford.edu
Wed Jun 6 16:39:43 EST 2001
Has anyone had experience with leaf clearing for microscopy? We started
with a published procedure for clearing grape leaves. The published
procedure uses 10% Na hypochlorite to destroy chlorophyll and other
pigments, followed by washing in water, dehydration with EtOH, switching to
xylenes, and mounting in Permount. We've been using Clorox, however,
(about 5% Na hypo). We find that our leaf pieces usually (but not always)
begin to disintegrate when transferred from bleach to water.
Any suggestions welcome.
Dr. Gary Coté
Department of Biology
Radford, VA 24142-6931
email: gcote at radford.edu
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