Leaf clearing for microscopy

David W. Kramer kramer.8 at osu.edu
Thu Jun 7 07:30:22 EST 2001


You can find several methods in Ruzin, Steven E.  1999.  Plant
Microtechnique and Microscopy.  New York:  Oxford University Press.
Several clearing methods are on pages 127-130.  All are fully documented
with references to the original literature where you might get additional
tips on your specific problem.


>Greetings,
>Has anyone had experience with leaf clearing for microscopy?  We started
>with a published procedure for clearing grape leaves.  The published
>procedure uses 10% Na hypochlorite to destroy chlorophyll and other
>pigments, followed by washing in water, dehydration with EtOH, switching to
>xylenes, and mounting in Permount.  We've been using Clorox, however,
>(about 5% Na hypo).  We find that our leaf pieces usually (but not always)
>begin to disintegrate when transferred from bleach to water.
>
>Any suggestions welcome.
>
>Gary
>
>Dr. Gary Coté
>Assistant Professor
>Department of Biology
>Box 6931
>Radford University
>Radford, VA 24142-6931
>
>Ph: 540-831-5630
>Fax: 540-831-5129
>email: gcote at radford.edu
>http://www.radford.edu:8800/~gcote/
>
>---

*********************
David W. Kramer, Chair
Education Committee
Botanical Society of America
http://www.botany.org

Asst. Prof. of Evolution, Ecology, and Organismal Biology
Ohio State University at Mansfield
1680 University Drive
Mansfield, OH  44906-1547
Phone:  (419) 755-4344      FAX:  (419) 755-4367
e-mail:  kramer.8 at osu.edu

---




More information about the Plant-ed mailing list