Root nodules

[Artus, Nancy N.]

I have also had bad luck with Carolina's inoculum.  You can dig up a clover
outside, remove and surface-sterilize some nodules, crush them in sterile
0.2 M sucrose (or water), and streak on yeast-mannitol agar.  In less
than a
week, you will have your own inoculum.  Then you can do experiments like
comparing +/- N fertilizer on nodule growth.

Nancy Artus
West Chester University

> ----------
> From: 	pelkki at svsu.edu
> Sent: 	Thursday, October 4, 2001 5:16 PM
> To: 	plant-ed at net.bio.net
> Subject: 	Root nodules
> 
> Hi, all!
> 
> I've been trying to grow clover, soybean, etc. to show root nodules to
> students without success.  Can anyone recommend a plant that does well
> and what is the amount of time needed to grow these plants from seeds in
> order to see root nodules?  I've been using the rhizobium innoculum from
> Carolina Biological... it's a black dust.
> 
> Thanks!
> Kathy Pelkki
> pelkki at svsu.edu
> 
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> 

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