Fwd: sudan III

David W. Kramer kramer.8 at osu.edu
Wed Feb 13 10:39:34 EST 2002


>Can anyone give information about what is sudan III? And how is useful?
>
>---
Here are some informational web sites but I am not certain they supply the
information you need:

A food component lab exercise (one of the tests is for lipids using Sudan
III) is at
http://gened.emc.maricopa.edu/bio/BIO102/biolabs/OrganicMols.html

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These instructions for preparing the Sudan stains is from
http://www.ops.org/curriculum/science/page6.htm

Sudan III: To make 100 ml of Sudan IV staining solution: warm 73.5 ml of
95% ethyl alcohol [C2H5OH] in a warm water bath (hot tap water will do).
Add 0.5 grams of Sudan (III) and stir. Add 75-80 degrees Celsius distilled
water to just below the 100 ml mark; stir. Cool to room temperature; dilute
to 100 ml with distilled water; stir then filter. (Use: biological stain)

 Sudan IV: To make 100 ml of Sudan IV staining solution: warm 75 ml of 95%
ethyl alcohol [C2H5OH] in a warm water bath (hot tap water will do). Add
0.5 grams of Sudan IV and stir. Cool to room temperature; dilute to 100 ml
with distilled water; stir then filter. (Use: biological stain)

*************************

Another lab exercise is at
http://www.csupomona.edu/~srkoukol/BIO115L/formallab.html

*********************
David W. Kramer, Ph.D.
Asst. Prof. of Evolution, Ecology, and Organismal Biology
Ohio State University at Mansfield
1680 University Drive
Mansfield, OH  44906-1547
Phone:  (419) 755-4344      FAX:  (419) 755-4367
e-mail:  kramer.8 at osu.edu

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