Reducing activity in cauliflower microsomes
gcote at radford.edu
Fri May 10 14:15:29 EST 2002
I have been using cauliflower as a source of mitos for succinate DH assay.
They work very well. However, I've also tried to assay the leftover
microsomes in order to demonstrate organelle-specific enzymes. Problem is
that the microsomes have a powerful, succinate-independent reducing
activity that can mask and mimic succinate DH (except of course for being
Does anyone know what this reducing activity may be? Or how to inhibit it
or eliminate it?
Alternatively, it could be a very interesting enzyme to look at in its own
right, if I had any idea what it was.
Dr. Gary Coté
Department of Biology
Radford, VA 24142-6931
email: gcote at radford.edu
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