Reducing activity in cauliflower microsomes

Gary Cote gcote at radford.edu
Fri May 10 14:15:29 EST 2002


I have been using cauliflower as a source of mitos for succinate DH assay.
They work very well.  However, I've also tried to assay the leftover
microsomes in order to demonstrate organelle-specific enzymes.  Problem is
that the microsomes have a powerful, succinate-independent reducing
activity that can mask and mimic succinate DH (except of course for being
succinate-independent).

Does anyone know what this reducing activity may be?  Or how to inhibit it
or eliminate it?

Alternatively, it could be a very interesting enzyme to look at in its own
right, if I had any idea what it was.

Gary Cote
Dr. Gary Coté
Assistant Professor
Department of Biology
Box 6931
Radford University
Radford, VA 24142-6931

Ph: 540-831-5630
Fax: 540-831-5129
email: gcote at radford.edu
http://www.radford.edu:8800/~gcote/

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