germinating fresh pollen

John Hewitson john_hewitson at breathe.com
Fri Feb 28 02:40:28 EST 2003


"David Fisher" wrote on 27/2/03 3:47 am

>
>
>Does anyone have a good protocol for making live pollen germinate for a
>general botany lab exercise?  The usual procedure, using Impatiens,
>rarely works.  Any secrets, anyone? David Fisher, PhD
>
>---
>

There is a protocol on the Science and Plants website which often gives 
good results and many teachers in UK swear by it at:-

www-saps.plantsci.cam.ac.uk/worksheets/ssheets/ssheet4.htm

It contains a link:-

http://www.microscopy-uk.org.uk/mag/ctpollen/Spring_flowers_and_pollen_germ
ination.html

to a very simple technique growing pollen on onion epidermis which I 
found to work as well as the SAPS protocol with 12 year old pupils (and 
that's a REAL test of any technique!)  This same website contains a bank 
of relevant photographs.

The SAPS protocol also includes a reference to a paper where the author 
surveyed the success he had with different species at different times of 
the year (with a link to a .pdf copy on the SAPS website).

I'd be interested to know of other's experiences.

John



Dr. John Hewitson
Science and Plants for Schools (SAPS)
Homerton College
Cambridge CB2 2PH  UK
email: hom-saps at lists.cam.ac.uk
Tel: +44 (0)1223 507168
Fax: +44 (0)1223 215004
web site: http://www-saps.plantsci.cam.ac.uk

---



More information about the Plant-ed mailing list