Protein from plant cells

Gary Cote gcote at radford.edu
Fri Jun 27 13:43:16 EST 2003


Has anyone tried extracting total protein for electrophoresis from plant 
cells?  We ground cells in SDS/ mercaptoethanol/ glycerol/ tris buffer
with 
a hand homogenizer, but found absolutely no bands on SDS PAGE.  We are 
wondering how this is possible.

Gary Coté

Dr. Gary Coté
Assistant Professor
Department of Biology
Box 6931
Radford University
Radford, VA 24142-6931

Ph: 540-831-5630
Fax: 540-831-5129
email: gcote at radford.edu
http://www.radford.edu:8800/~gcote/

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