John E. Silvius
SILVIUSJ at cedarville.edu
Wed Apr 28 14:22:59 EST 2004
I cannot speak for RNA yield, but my experience with liquid N2-frozen
leaf tissue stored for a number of weeks, then lyophilized, gives good
enzyme activity, reflective of direct extractions from fresh tissue.
Professor of Biology
>>> "Robinson, Dr. David" <drobinson at bellarmine.edu> 4/28/2004 11:12:11
I know this is not exactly a "Plant Education" question (except that
involves student research).....but does anyone know whether we can
plant tissue (by quick-freezing it in liquid nitrogen, and then
it at -80C) and still get a good yield of mRNA?
I have heard stories that it is far better to work with fresh tissue,
but wondering if I can "get by" storing it like this for awhile.
Thanks for any advice!=20
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