mRNA extraction

Dr. Cindy Graham cmgraham at ucalgary.ca
Wed Apr 28 15:26:43 EST 2004


If you lyophilize plant tissue the RNA will degrade.  However, you can 
use lyophilized plant tissue for DNA extraction.


On Apr 28, 2004, at 1:22 PM, John E. Silvius wrote:

> Dave,
> I cannot speak for RNA yield, but my experience with liquid N2-frozen
> leaf tissue stored for a number of weeks, then lyophilized, gives good
> enzyme activity, reflective of direct extractions from fresh tissue.
>
> John Silvius
> Professor of Biology
> Cedarville University
>
>>>> "Robinson, Dr. David" <drobinson at bellarmine.edu> 4/28/2004 11:12:11
> AM >>>
>
> I know this is not exactly a "Plant Education" question (except that
> it
> involves student research).....but does anyone know whether we can
> store
> plant tissue (by quick-freezing it in liquid nitrogen, and then
> storing
> it at -80C) and still get a good yield of mRNA?
> =20
> I have heard stories that it is far better to work with fresh tissue,
> but wondering if I can "get by" storing it like this for awhile.
> =20
> Thanks for any advice!=20
>
> Dave Robinson
> Biology Department
> Bellarmine University
> 2001 Newburg Road
> Louisville, KY  40205
>
> 502-452-8197
> ---
>
Dr. Cindy Graham,
Instructor - Natural Sciences Program
Department of Biological Sciences
University of Calgary
2500 University Dr. NW
Calgary, AB.
T2N 1N4
(403) 210-8822

---



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