[Plant-education] No Adeventitious Roots!

William E Williams WEWilliams at smcm.edu
Fri Feb 24 09:55:58 EST 2006


Dear Plant-Eders,

We've been doing a mung-bean adventitious-root experiment for years  
now in our first-year biology course, and this year for the first  
time we have NO adventitious roots, even in the water control  
(usually it develops a few very long adventitious roots, quite  
according to Hoyle. Or perhaps Went.). We do see some plant death in  
the highest auxin concentrations (we usually do, so this suggests  
that our auxin is active), but not one single jar has even a hint of  
an adventitious root.

Suggestions? Only thing I can think of is that there's something  
peculiar with the water -- we did have an E. coli alarm a few weeks  
ago and so they spiked our well with chlorine, but we used RO water  
with a substantial charcoal filter on the end of the RO machine,  
which should have removed most of the chlorine.

We're stumped. I thought I remembered a posting here a while ago  
about some plant species that had had auxin response bred out of  
them, but I couldn't find it in the archives; are maybe the newest  
mung-bean seeds are not auxin responsive? But then, why wouldn't we  
have roots on the control?

-W2

William E. Williams <mailto:WEWilliams at smcm.edu>
Professor of Biology
Saint Mary's College of Maryland
18952 E Fisher Rd, Saint Marys City, MD 20686
(240)895-4365



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